Selection of a Suitable Medium to Determine Sensitivity of Monilinia fructicola Mycelium to SDHI Fungicides
Selection of a Suitable Medium to Determine Sensitivity of Monilinia fructicola Mycelium to SDHI Fungicides
复制标题
选择合适的培养基来确定Monilinia fructicola 菌丝体对 SDHI 杀菌剂的敏感性
DOI：
10.1111/j.1439-0434.2011.01815.x
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发表时间：
2011-09-01
影响因子：
1.5
通讯作者：
Schnabel, Guido
中科院分区：
文献类型：
--
作者：
Hu, Meng-Jun;Luo, Chao-Xi;Schnabel, Guido
琥珀酸脱氢酶抑制剂（SDHI）是防治核果类水果采前和采后褐腐病的重要药剂。在这项研究中，我们确定了各种媒体上的菌丝生长速率的Monilinia fructicola分离物，并挑选了三个允许最快的增长，以评估菌丝体的敏感性SDHI杀菌剂啶酰菌胺，氟吡菌酰胺，吡噻菌胺。基本培养基（MM）支持菌丝生长最好，并产生最低的EC50值的三个SDHI杀菌剂。EC 50值与从离体果实测定中获得的发病率数据相对应。在α = 0.05水平下，吡噻菌胺的体外固有活性显著高于氟吡菌酰胺，在α = 0.1水平下，吡噻菌胺的体外固有活性显著高于啶酰菌胺。然而，离体果实试验表明，这种“优势”在体内并没有延续。总之，MM似乎是目前可用于评估M敏感性的最佳培养基。果霉菌丝体的体外培养。
Succinate dehydrogenase inhibitor (SDHI) fungicides are important tools to control preharvest and post-harvest brown rot of stone fruits. In this study we determined the mycelial growth rate of Monilinia fructicola isolates on various media and picked the three that allowed the fastest growth to assess the sensitivity of mycelium to SDHI fungicides boscalid, fluopyram, and penthiopyrad. Minimal medium (MM) supported mycelial growth the best and yielded lowest EC50 values for three SDHI fungicides. EC50 values corresponded with disease incidence data obtained from detached fruit assays. Penthiopyrad had significantly greater intrinsic activity in vitro compared to fluopyram at the alpha = 0.05 level and compared to boscalid at the alpha = 0.1 level. However, detached fruit assays revealed that this 'advantage' did not carry over in vivo. In conclusion, MM appears to be the best medium currently available to assess the sensitivity of M. fructicola mycelium in vitro.