Noncoordinated expression of α-, β-, and γ-subunit mRNAs of epithelial Na+ channel along rat respiratory tract
Noncoordinated expression of α-, β-, and γ-subunit mRNAs of epithelial Na+ channel along rat respiratory tract
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大鼠呼吸道上皮Na+通道α-、β-和γ-亚基mRNA的不协调表达
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
J. Bonvalet
中科院分区：
文献类型：
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作者：
N. Farman;C. Talbot;R. Boucher;M. Fay;C. Canessa;B. Rossier;J. Bonvalet
呼吸道上皮表面的Na +重吸收在呼吸生理中起着重要作用。与肾集合小管上皮和远端结肠上皮一样，Na +通过最近克隆的对阿米洛利敏感的多亚单位（α, β, γ）上皮Na +通道进入呼吸上皮细胞的管腔表面。我们利用原位杂交技术检测了大鼠肺和上气道上皮细胞中大鼠上皮Na +通道（rENaC）的α-、β-和γ-亚基在mRNA水平上的表达。在气管上皮、肺泡细胞亚群（可能是II型肺细胞）以及鼻腺和气管腺腺中，α-和γ-rENaC亚基表达（超过β）的发生率很高。相比之下，在细支气管上皮和大鼠鼻腺导管中检测到这三种亚基的表达水平相同。这种表达的多样性可能反映了沿呼吸道的amiloride敏感Na +通道的细胞特异性功能。
Na + reabsorption from the epithelial surface of the respiratory tract plays a fundamental role in respiratory physiology. As in the epithelia of the renal collecting tubule and distal colon, Na + enters across the luminal surface of respiratory epithelial cells via a recently cloned amiloride-sensitive multisubunit (α, β, γ) epithelial Na + channel. We have examined the cellular expression at the mRNA level of the α-, β-, and γ-subunits of rat epithelial Na + channel (rENaC) in the rat lung and upper airway epithelial cells using in situ hybridization. A large prevalence of α- and γ-rENaC subunit expression (over β) was found in tracheal epithelium, in a subpopulation of alveolar cells, presumably type II pneumocytes, and in nasal and tracheal gland acini. In contrast, equivalent levels of expression of all three subunits were detected in bronchiolar epithelium and in rat nasal gland ducts. This diversity of expression may reflect cell-specific functions of the amiloride-sensitive Na + channel along the respiratory tract.