Electrokinetic Perfusion Through Three-Dimensional Culture Reduces Cell Mortality
Electrokinetic Perfusion Through Three-Dimensional Culture Reduces Cell Mortality
复制标题
通过三维培养的动电灌注降低细胞死亡率
DOI：
10.1089/ten.tea.2021.0008
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发表时间：
2021
影响因子：
4.1
通讯作者：
Messerli, Mark A.
中科院分区：
文献类型：
--
作者：
Sarkar, Anyesha;Messerli, Mark A.
细胞的增殖和存活依赖于质传递。在体内，流体流动促进了通过脉管系统和间质空间的质量传递，提供了营养物质的持续供应和细胞废物的清除。在缺乏足够流量的情况下，质传递受到扩散的限制，并对组织工程、组织移植和退行性疾病治疗中的细胞存活构成重大挑战。人工灌注可以克服这些挑战。在这项工作中，我们比较了压力驱动灌注（PDP）和电动灌注（EKP）在降低三维培养的Matrigel细胞外基质细胞死亡率方面的效果。我们通过Matrigel表征电渗透流动，以确定产生与压力诱导的间隙流速相似的条件。我们还比较了连续或脉冲EKP诱导的细胞死亡率的变化。我们报道，在相同流速下，连续EKP比PDP更能显著降低整个灌注通道的死亡率，并且脉冲EKP与连续EKP一样有效地降低死亡率。我们得出结论，在促进新生血管和血管生成前的组织存活方面，EKP比PDP具有显著的优势。影响陈述间质流动有助于促进组织和器官的质量传递和细胞存活。本研究在三维培养中使用压力驱动灌注（PDP）或电动灌注（EKP）产生间质流动，以促进细胞活力。与PDP相比，通过带电荷的细胞外基质进行EKP具有显著的优势，并可能在组织工程、移植和退行性疾病的治疗中促进细胞存活。
Cell proliferation and survival are dependent on mass transfer.In vivo, fluid flow promotes mass transfer through the vasculature and interstitial space, providing a continuous supply of nutrients and removal of cellular waste products. In the absence of sufficient flow, mass transfer is limited by diffusion and poses significant challenges to cell survival during tissue engineering, tissue transplantation, and treatment of degenerative diseases. Artificial perfusion may overcome these challenges. In this work, we compare the efficacy of pressure driven perfusion (PDP) with electrokinetic perfusion (EKP) toward reducing cell mortality in three-dimensional cultures of Matrigel extracellular matrix. We characterize electro-osmotic flow through Matrigel to identify conditions that generate similar interstitial flow rates to those induced by pressure. We also compare changes in cell mortality induced by continuous or pulsed EKP. We report that continuous EKP significantly reduced mortality throughout the perfusion channels more consistently than PDP at similar flow rates, and pulsed EKP decreased mortality just as effectively as continuous EKP. We conclude that EKP has significant advantages over PDP for promoting tissue survival before neovascularization and angiogenesis.Impact statementInterstitial flow helps promote mass transfer and cell survival in tissues and organs. This study generated interstitial flow using pressure driven perfusion (PDP) or electrokinetic perfusion (EKP) to promote cell viability in three-dimensional cultures. EKP through charged extracellular matrices possesses significant advantages over PDP and may promote cell survival during tissue engineering, transplantations, and treatment of degenerative diseases.