Cancer cells responsible for humoral hypercalcemia express mRNA encoding a secreted form of ODF/TRANCE that induces osteoclast formation
Cancer cells responsible for humoral hypercalcemia express mRNA encoding a secreted form of ODF/TRANCE that induces osteoclast formation
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DOI：
10.1006/bbrc.2000.2314
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发表时间：
2000-03-16
影响因子：
3.1
通讯作者：
Sato, N
中科院分区：
文献类型：
--
作者：
Nagai, M;Kyakumoto, S;Sato, N
一种新的分泌型破骨细胞分化因子/肿瘤坏死因子相关激活诱导细胞因子的cDNA（sODF/TRANCE，GenBank Accession No.AB037599）从鳞状细胞癌细胞系SCC-4和T3 M-1C1.2的5 ′ RACE cDNA克隆测序，其中亲本恶性肿瘤为SCC-4和T3 M-1C1.2; sODF/TRANCE cDNA由未知的5'端序列和100%同源的ODF/TRANCE胞外区编码区序列组成。新cDNA的最长开放阅读框（ORF）与ODF/TRANCE cDNA编码胞外区C-末端氨基酸残基（74-318）的ORF的3'端完全匹配。在癌细胞调节的培养基中检测到与ODF/TRANCE胞外结构域特异性抗体反应的相应蛋白质。此外，人早幼粒细胞白血病细胞HL 60在SCC-4和T3 M-1 CL 2细胞的条件培养基中分化为破骨细胞样细胞（OCL）。抗ODF/TRANCE抗体可抑制HL 60细胞向OCL的分化。这些结果有力地表明，sODF/TRANCE在恶性肿瘤体液性高钙血症中增强骨吸收中起重要作用（C）2000学术出版社。
A novel cDNA encoding a secreted form of osteoclast differentiation factor/tumor necrosis factor-related activation-induced cytokine (sODF/TRANCE, GenBank Accession No. AB037599) was sequenced from 5' RACE cDNA clones of squamous cell carcinoma cell lines, SCC-4 and T3M-1 Cl.2, of which parental malignant tis;sues had caused severe humoral hypercalcemia The sODF/TRANCE cDNA was composed of unknown 5' end sequence followed by the 100% identical sequence of the ODF/TRANCE extracellular domain-coding region. The longest open reading frame (ORF) of the novel cDNA completely matched the 3' end of the ORF of the ODF/TRANCE cDNA encoding C-terminal amino acid residues (74-318) in the extracellular region. The corresponding protein that reacted with the antibody specific for the extracellular domain of ODF/TRANCE was detected in the culture media conditioned by the cancer cells. Furthermore, human promyeloblastic leukemia cells HL60, differentiated into osteoclast-like cells (OCLs) when cultured in the media conditioned by SCC-4 and T3M-1 CL2 cells. The differentiation of HL6O cells into OCLs was inhibited by the anti-ODF/TRANCE antibody. These results strongly suggest that sODF/TRANCE plays an important role in enhanced bone-resorption in humoral hgpercalcemia of malignancy (C) 2000 Academic Press.