Trait-based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer
Trait-based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer
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DOI：
10.1002/lno.11189
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发表时间：
2019-07-01
影响因子：
4.5
通讯作者：
Purdie, Duncan Alastair
中科院分区：
文献类型：
--
作者：
Fragoso, Glaucia Moreira;Poulton, Alex James;Purdie, Duncan Alastair
浮游生物是一个极其多样化和多系的群体，具有广泛的形态和生理特征。在这里，我们应用由脉冲形状记录自动流式细胞仪- cytosense提供的自动化光学技术来研究北大西洋亚极地北极和大西洋水域浮游植物和可塑性纤毛虫的性状变异性。我们使用了来自CytoSense的生物光学描述符（光散射[向前和向侧]和荧光[分别来自叶绿素a、降解色素和藻胆蛋白的红色、黄色/绿色和橙色]），并将它们转化为功能性状，以证明生态性状在环境梯度上的可变性。细胞大小是本研究中变化的主要特征，在北极水域中发现了大型光合微浮游生物（细胞直径约为20 μ m），包括单细胞和链状的硅藻，以及可塑性纤毛虫，而在大西洋水域中则以小型浮游植物类群为主，如微真核生物（< 4 μ m）和蓝藻共生球菌。形态特征，如链/集落形成和结构复杂性（即细胞过程、刚毛和内部液泡），似乎有利于高度光照和分层的北极水域的浮力。在大西洋水域，小的细胞尺寸和球形的细胞形状，加上光生理特性，如高度的内部色素沉着，为在大西洋低营养和动态混合的水域中生存提供了颜色适应。量化生态特征的自动化技术的使用为揭示浮游生物群落和海洋生态系统的结构和功能之间的联系提供了令人兴奋的新机会。
Plankton are an extremely diverse and polyphyletic group, exhibiting a large range in morphological and physiological traits. Here, we apply automated optical techniques, provided by the pulse-shape recording automated flow cytometer-CytoSense-to investigate trait variability of phytoplankton and plastidic ciliates in Arctic and Atlantic waters of the subpolar North Atlantic. We used the bio-optical descriptors derived from the CytoSense (light scattering [forward and sideward] and fluorescence [red, yellow/green and orange from chlorophyll a, degraded pigments, and phycobiliproteins, respectively]) and translated them into functional traits to demonstrate ecological trait variability along an environmental gradient. Cell size was the master trait varying in this study, with large photosynthetic microplankton (> 20 mu m in cell diameter), including diatoms as single cells and chains, as well as plastidic ciliates found in Arctic waters, while small-sized phytoplankton groups, such as the picoeukaryotes (< 4 mu m) and the cyanobacteria Synechococcus were dominant in Atlantic waters. Morphological traits, such as chain/colony formation and structural complexity (i.e., cellular processes, setae, and internal vacuoles), appear to favor buoyancy in highly illuminated and stratified Arctic waters. In Atlantic waters, small cell size and spherical cell shape, in addition to photo-physiological traits, such as high internal pigmentation, offer chromatic adaptation for survival in the low nutrient and dynamic mixing waters of the Atlantic Ocean. The use of automated techniques that quantify ecological traits holds exciting new opportunities to unravel linkages between the structure and function of plankton communities and marine ecosystems.