Two Plasmodium knowlesi-specific antigens on the surface of schizont-infected Rhesus monkey erythrocytes induce antibody production in immune hosts.
Two Plasmodium knowlesi-specific antigens on the surface of schizont-infected Rhesus monkey erythrocytes induce antibody production in immune hosts.
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DOI：
10.1084/jem.150.1.86
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发表时间：
1979-07-01
期刊：
影响因子：
--
通讯作者：
Lightholder J
中科院分区：
文献类型：
--
作者：
Schmidt-Ullrich R;Wallach DF;Lightholder J
用恒河猴抗疟原虫超免疫血清和天然免疫猴血清的免疫球蛋白，对来自马来西亚和菲律宾诺氏疟原虫株的纯化疟原虫（6- 10个核）和疟原虫感染红细胞膜进行免疫化学分析。抗抗体IG在Triton X-100溶解的抗体和感染细胞的膜中识别不到20种免疫组分。在这些抗原中，9（组分1、3、4、5、6、10、11、18和20）是寄生虫和受感染红细胞膜所共有的，和12（2A、B、6、8、9、12、13 p、14、16 A、B、19 A、Bp、21、22 p和23）主要存在于寄生虫中; 4种组分（13 i、19 A、Bi、22 A、B和24）是感染红细胞膜所特有的。只有三种寄生虫特异性组分（1、13和19）暴露在寄生的红细胞表面上，如乳过氧化物酶催化的放射性碘标记和使用完整感染的红细胞广泛吸收抗寄生虫IG所示。感染红细胞表面上的两种疟原虫特异性抗原（1和13）可被对诺氏疟原虫感染具有天然免疫力的恒河猴血清识别，因此代表体内抗原。当用两种类型的抗血清进行分析时，对诺氏疟原虫的两种不同菌株的寄生红细胞的寄生物和膜的分析仅产生一些微小的定量差异，但没有定性差异。重要的是，组分1和13在两种菌株中看起来相同。
Purified schizonts (6--10 nuclei) and membranes of schizont-infected erythrocytes from the Malaysian and Philippine strain of Plasmodium knowlesi are analyzed immunochemically using immunoglobulin of rhesus monkey hyperimmune sera against schizonts and of sera from naturally immune monkeys. The anti-schizont Ig identifies less than 20 immune components in Triton X-100-solubilized schizonts and membranes of infected cells. Of these antigens, 9 (component 1, 3, 4, 5, 6, 10, 11, 18, and 20) are common to parasites and membranes of infected erythrocytes, and 12 (2A,B, 6, 8, 9, 12, 13p, 14, 16A,B, 19 A,Bp, 21, 22p, and 23) are predominantly found in the parasite; 4 components (13i, 19A,Bi, 22A, B, and 24) are unique to the membrane of infected erythrocytes. Only three parasite-specific components (1, 13, and 19) are exposed on the surface of parasitized erythrocytes as revealed by both lactoperoxidase-catalyzed radioiodination and extensive absorption of anti-schizont Ig using intact infected erythrocytes. Two plasmodium- specific antigens (1 and 13) on the surface of infected erythrocytes are recognized by sera of rhesus monkeys rendered naturally immune against P. knowlesi infections and, therefore, represent antigens in vivo. Analyses of schizonts and membranes of parasitized erythrocytes of the two different strains of P. knowlesi yields only some minor quantitative, but no qualitative differences when analyzed with both types of antisera. Importantly, components 1 and 13 appear identical in both strains.