A heterochromatin protein 1 homologue in Caenorhabditis elegans acts in germline and vulval development
A heterochromatin protein 1 homologue in Caenorhabditis elegans acts in germline and vulval development
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DOI：
10.1093/embo-reports/kvf051
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发表时间：
2002-03-01
期刊：
影响因子：
7.7
通讯作者：
Palladino, F
中科院分区：
文献类型：
--
作者：
Couteau, F;Guerry, F;Palladino, F
已发现高度保守的异染色质蛋白1（HP 1）家族的蛋白质在整个真核生物界中在核结构的动态组织和基因调控中起作用。除了在异染色质介导的基因沉默的关键球员，HP 1蛋白也可能有助于通过招募到特定的启动子的常染色质基因的转录抑制。为了研究这些不同的活动在特定的发育途径中所起的作用，我们确定了秀丽隐杆线虫基因组中的HP 1同源物，并使用RNA介导的干扰来研究它们的功能。我们表明，同源物之一，HPL-2，是需要一个功能性生殖系的形成和外阴的发展，通过在Rb相关的途径行事。我们认为，作为基因表达的阻遏物，HP 1蛋白可以履行特定的功能，在整个发展过程中的体细胞和生殖细胞分化过程。
Proteins of the highly conserved heterochromatin protein 1 (HP1) family have been found to function in the dynamic organization of nuclear architecture and in gene regulation throughout the eukaryotic kingdom. In addition to being key players in heterochromatin-mediated gene silencing, HP1 proteins may also contribute to the transcriptional repression of euchromatic genes via the recruitment to specific promoters. To investigate the role played by these different activities in specific developmental pathways, we identified HP1 homologues in the genome of Caenorhabditis elegans and used RNA-mediated interference to study their function. We show that one of the homologues, HPL-2, is required for the formation of a functional germline and for the development of the vulva by acting in an Rb-related pathway. We suggest that, by acting as repressors of gene expression, HP1 proteins may fulfil specific functions in both somatic and germline differentiation processes throughout development.