Assessment of three automated assays for C-reactive protein determination in dogs
Assessment of three automated assays for C-reactive protein determination in dogs
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DOI：
10.2460/ajvr.68.12.1281
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发表时间：
2007-12-01
影响因子：
1
通讯作者：
Hagman, Ragnvi
中科院分区：
文献类型：
--
作者：
Fransson, Boel A.;Bergstrom, Annika;Hagman, Ragnvi
目的：探讨犬C-反应蛋白自动测定仪的特点，并对两种人C-反应蛋白测定仪在犬身上的应用进行评价。56只患子宫积脓的客户犬和11只健康对照犬。11只犬的CBP含量高(>；100 mg/L)或低(<；10 mg/L)(用犬的EL ISA法测定)。通过使用这两个血浆池来确定试剂盒内和试剂盒间的不精密度，并通过对通过ELISA法和犬血清白蛋白自动测定仪获得的结果进行Logistic回归分析来评估试剂盒的不精密度。结果：采用双抗体夹心法测定10只犬的血浆CBP浓度。结果：用双抗体夹心酶联免疫吸附试验测定，子宫积液组和正常对照组犬血浆CBP平均浓度分别为96.1+/-38.5 mg/L和10.1+/-23.2 mg/L。犬自动化检测的批内变异系数(CVs)分别为78%和79%，批间变异系数(CVs)分别为11.1%和13.1%。自动检测的结果与通过酶联免疫吸附试验获得的结果高度相关。人测结果均未超过0.4 mg/L。结论与临床意义--犬C反应蛋白全自动测定法与酶联免疫吸附试验相比，具有较低的检定间不精密度。两种人C反应蛋白检测方法均不适用于犬血浆标本的分析。犬全自动C-反应蛋白测定法比酶联免疫吸附试验更准确地测定犬血浆C-反应蛋白浓度。
Objective-To determine the characteristics of an automated canine C-reactive protein (CRP) assay and evaluate 2 human CRP assays for use in dogs.Animals-56 client-owned dogs with pyometra and 11 healthy control dogs.Procedures-Samples from 11 dogs with high (> 100 mg/L) or low (< 10 mg/L) CBP concentrations (determined by use of a canine ELISA) were evaluated by use of the automated canine CBP assay. Intra- and interassay imprecision was determined (by use of those 2 plasma pools), and assay inaccuracy was assessed by use of logistic regression analysis of results obtained via ELISA and the automated canine CRP assay. Two automated human CBP assays were used to measure plasma CBP concentration in 10 dogs.Results-By use of the ELISA, mean +/- SD plasma CBP concentration was 96.1 +/- 38.5 mg/L and 10.1 +/- 23.2 mg/L in dogs with pyometra and control dogs, respectively. The automated canine assay had intra-assay coefficients of variation (CVs) of 78% and 79%, respectively, and interassay CVs of 11.1 % and 13.1 %, respectively. Results from the automated assay were highly correlated with results obtained via ELISA. The human assay results did not exceed 0.4 mg/L in any dog.Conclusions and Clinical Relevance-The automated canine CRP assay had less interassay imprecision, compared with the ELISA. The 2 human CRP assays were not suitable for analysis of canine plasma samples. The automated canine CRP assay was more precise than the ELISA for serial evaluations of plasma CRP concentration in dogs.