Epidemiological and serological survey of brucellosis in mongolia by ELISA using sarcosine extracts
Epidemiological and serological survey of brucellosis in mongolia by ELISA using sarcosine extracts
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DOI：
10.1111/j.1348-0421.2004.tb03553.x
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发表时间：
2004-01-01
影响因子：
2.6
通讯作者：
Makino, S
中科院分区：
文献类型：
--
作者：
Erdenebaatar, J;Bayarsaikhan, B;Makino, S
布鲁氏菌病是一种重要的人畜共患病，血清学监测是控制布鲁氏菌病的关键。但牛种布鲁氏菌S-19株和B株的减毒活细胞间存在交叉反应。羊种菌株Rev-1与小肠结肠炎耶尔森氏菌09或接种动物血清的混合物会干扰虎红试验（RBT）的准确血清学诊断。因此，我们使用ELISA与肌氨酸提取物从剧毒B。牛流产杆菌544株，以消除RBT阳性血清中的假阳性。在蒙古，从23个游牧牛群的人和动物中共采集了697份血清样本。牛群被分为三组：布鲁氏菌病流行（BE）、疑似布鲁氏菌病（BS）或布鲁氏菌病疫苗接种（BV）。295只动物（43.0%）通过RBT呈阳性，但其中206只（69.8%）根据ELISA呈阳性;因此，发现30.2%的RBT阳性血清为假阳性。RTB的假阳性样本分别占BE、BS和BV畜群动物的4.1%、27.4%和68.2%。此外，32%的RBT阳性人血清也是假阳性。因此，我们的ELISA将比RTB更特异，并用于布鲁氏菌病的流行病学监测。
Brucellosis is an important zoonosis, and serological surveillance is essential to its control. However, cross-reactions of attenuated live cells of Brucella abortus strain S-19 and B. melitensis strain Rev-1 with Yersinia enterocolitica 09 or vaccinated animal sera interfere with accurate serological diagnosis by the Rose Bengal test (RBT). Therefore, we used ELISA with sarcosine extracts from the virulent B. abortus strain 544 to eliminate false-positives among RBT positive-sera. A total of 697 serum samples were collected in Mongolia from humans and animals in 23 nomadic herds. The herds were classified into three groups as brucellosis-endemic (BE), brucellosis-suspected (BS), or Brucella-vaccinated (BV). The number of 295 animals (43.0%) was positive by RBT, but 206 (69.8%) of these were positive according to ELISA; therefore, 30.2% of the RBT-positive sera were found to be false positives. The false positive samples for RTB represent 4.1%, 27.4%, and 68.2% of the animals from the BE, BS, and BV herds, respectively. In addition, 32% of RBT-positive human sera were also false positives. Thus, our ELISA would be more specific than RTB and useful for epidemiological surveillance for brucellosis.