A method allowing intracellular and extracellular single-unit recordings from brain slices in the grease-gap chamber.
A method allowing intracellular and extracellular single-unit recordings from brain slices in the grease-gap chamber.
复制标题
一种允许从油脂间隙室中的脑切片进行细胞内和细胞外单个单元记录的方法。
DOI：
10.1016/0165-0270(94)00153-8
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发表时间：
1995
影响因子：
3
通讯作者：
Wong,RK
中科院分区：
文献类型：
--
作者：
Stewart,M;Wong,RK
油脂间隙腔室使得分别灌注脑切片的两个部分成为可能。因此，就接受受控药物剂量的区域的大小而言，它位于浴施用和离子电渗疗法或压力喷射之间。修改的市售的腔室进行了描述，允许第一个细胞外和细胞内的单单位记录采取从脑切片中的油脂间隙准备。此外，我们描述的电阻的差距作为一个衡量的完整性的障碍和一个简单的方法，用于连续监测这个电阻。阻力监测器在低流速条件下特别有用，可提高机械稳定性。这些技术将脂隙法扩展到切片中单细胞的电生理学研究。
The grease-gap chamber makes it possible to perfuse two parts of a brain slice separately. Hence, it lies between bath application and iontophoresis or pressure ejection in terms of the size of the region that receives controlled drug dosing. Modifications of a commercially available chamber are described which have permitted the first extracellular and intracellular single-unit recordings to be taken from brain slices in a grease-gap preparation. In addition, we describe the electrical resistance of the gap as a measure of the integrity of the barrier and a simple method for monitoring this resistance continuously. The resistance monitor is particularly useful during low flow rate conditions that improve mechanical stability. These techniques extend the grease-gap method to electrophysiological studies of single cells in slices.