The absolute configuration of biotin.
The absolute configuration of biotin.
复制标题
生物素的绝对构型。
DOI：
10.1021/bi00866a044
复制
发表时间：
1966
期刊：
影响因子：
2.9
通讯作者：
J. Hamilton
中科院分区：
文献类型：
--
作者：
J. Trotter;J. Hamilton
通过x射线晶体分析（Bonnemere et al., 1965）确定了二氧化碳生物素的双对溴酰胺的结构，并且，与此类分析中通常的一样，仅获得了生物素每个不对称中心的相对构型。该结果与未取代生物素的x射线研究结果非常一致，其中也只确定了相对构型（Traub， 1956年和personal communication， 1964年）。然而，通过仔细测量x射线的异常色散（Bijvoet et al., 1951）来确定绝对构型是可能的，本文描述了一系列对生物素的双对溴苯胺的测量，从而建立了生物素的绝对立体化学。根据最终的位置和各向同性热参数计算了c02 -生物素衍生物的所有hkl的Idd反射的结构因子
TJLhe structure of the bis-p-bromoamlide of carbon dioxide biotin has been determined by X-ray crystallographic analysis (Bonnemere et al., 1965), and, as is usual in this type of analysis, only the relative con-figuration at each of the asymmetric centers of biotin was obtained. The results are in goodagreement with those obtainedfrom an X-ray study of unsubstituted biotin, where again only the relative configurations were determined (Traub, 1956, and personal communication, 1964). It is, however, possible, by careful meas-urement of the anomalous dispersion of X-rays (Bijvoet et al., 1951), todetermine the absolute configuration, and the present paper describes a series of such meas-urements for the bis-p-bromoanilide of COrbiotin, which establishes the absolute stereochemistry of biotin. Structure factors were calculated for all the hkl the Idd reflections of the C02-biotin derivative from the final positional and isotropic thermal parameters