AN RNA POLYMERASE-II TRANSCRIPTION FACTOR BINDS TO AN UPSTREAM ELEMENT IN THE ADENOVIRUS MAJOR LATE PROMOTER
AN RNA POLYMERASE-II TRANSCRIPTION FACTOR BINDS TO AN UPSTREAM ELEMENT IN THE ADENOVIRUS MAJOR LATE PROMOTER
复制标题
DOI：
10.1016/0092-8674(85)90174-6
复制
发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
SHARP, PA
中科院分区：
文献类型：
--
作者：
CARTHEW, RW;CHODOSH, LA;SHARP, PA
凝胶电泳DNA结合试验已被用于鉴定HeLa细胞提取物中特异性结合腺病毒主要晚期启动子的蛋白质。一个主要的晚期启动子转录因子MLTF已被检测为离散的蛋白质-DNA复合物。MLTF与主要晚期启动子的TATA盒上游序列特异性结合并具有高亲和力。该因子在DNA酶足迹分析中保护17 bp（-50至-66）区域。已证明同一区域对于体内和体外主要晚期启动子的有效转录都很重要。MLTF刺激体外转录只从模板含有这个上游区域。通过两个色谱步骤的MLTF cofractile的结合、足迹和转录刺激活性。这些结果表明MLTF与上游元件的直接结合激活了主要晚期启动子的转录。
A gel electrophoresis DNA binding assay has been used to identify proteins in the HeLa cell extracts that specifically bind to the major late promoter of adenovirus. A major late promoter transcription factor MLTF has been detected as a discrete protein-DNA complex. MLTF binds specifically and with high affinity to sequences upstream of the TATA box of the major late promoter. This factor protects a 17 bp (-50 to -66) region in a DNAase footprinting assay. The same region has been shown to be important for efficient transcription from the major late promoter both in vivo and in vitro. MLTF stimulates in vitro transcription only from a template containing this upstream region. The binding, footprinting, and transcription-stimulatory activities of MLTF cofractionate through two chromatographic steps. These results suggest that direct binding of MLTF to an upstream element activates transcription from the major late promoter.