MPScope: A versatile software suite for multiphoton microscopy
MPScope: A versatile software suite for multiphoton microscopy
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DOI：
10.1016/j.jneumeth.2006.03.001
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发表时间：
2006-09-30
影响因子：
3
通讯作者：
Kleinfeld, David
中科院分区：
文献类型：
--
作者：
Nguyen, Quoc-Thang;Tsai, Philbert S.;Kleinfeld, David
MPScope是一个软件套件，用于控制和分析来自定制多光子激光扫描荧光显微镜的数据。采集程序MPScan从多达四个成像通道和多达两个模拟输入采集、显示和存储电影、线条扫描、图像堆叠或任意区域，同时实时绘制感兴趣区域的强度。使用典型的检流计扫描仪，双向线阵扫描仪允许以高达10fps的速度采集256 x 256像素的帧。快速堆叠模式将电影采集与连续的z焦点运动和调整激光强度相结合，以实现恒定的图像亮度。快速堆叠可以通过在集成脚本环境中运行的定制程序实现自动化，允许在大约1小时内以10亿个体素对1 mm(3)皮质体积进行采样。分析程序MPView允许查看存储的帧、投影、自动检测细胞和绘制其跨帧的平均强度、直接将帧传输到Matlab、创建AVI电影和将文件导出到ImageJ。优化代码、多线程和COM(通用对象模型)技术的结合使MPScope能够充分利用定制的双光子显微镜，并简化它们的实现。(C)2006爱思唯尔B.V.保留所有权利。
MPScope is a software suite to control and analyze data from custom-built multiphoton laser scanning fluorescence microscopes. The acquisition program MPScan acquires, displays and stores movies, linescans, image stacks or arbitrary regions from up to four imaging channels and up to two analog inputs, while plotting the intensity of regions of interest in real-time. Bidirectional linescans allow 256 x 256 pixel frames to be acquired at up to 10 fps with typical galvanometric scanners. A fast stack mode combines movie acquisition with continuous z-focus motion and adjustment of laser intensity for constant image brightness. Fast stacks can be automated by custom programs running in an integrated scripting environment, allowing a 1 mm(3) cortical volume to be sampled in 1 billion voxels in approximately 1 h. The analysis program MPView allows viewing of stored frames, projections, automatic detection of cells and plotting of their average intensity across frames, direct frame transfer to Matlab, AVI movie creation and file export to ImageJ. The combination of optimized code, multithreading and COM (Common Object Model) technologies enables MPScope to fully take advantage of custom-built two-photon microscopes and to simplify their realization. (c) 2006 Elsevier B.V. All rights reserved.