In vivo quantitative analysis of the effect of hydration (immersion and Vaseline treatment) in skin layers using high-resolution MRI and magnetisation transfer contrast
In vivo quantitative analysis of the effect of hydration (immersion and Vaseline treatment) in skin layers using high-resolution MRI and magnetisation transfer contrast
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DOI：
10.1111/j.1600-0846.2004.00047.x
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发表时间：
2004-02-01
影响因子：
2.2
通讯作者：
Sharp, JC
中科院分区：
文献类型：
--
作者：
Mirrashed, F;Sharp, JC
背景/目的：关于外用制剂在保湿皮肤方面的有效性，人们提出了许多说法，但这些说法中的大多数都无法通过对角质层以下皮肤层的科学研究来证实，也没有关于剩余的表皮和真皮的信息。这证明了一种体内定量方法来测量水分负荷对皮肤各层的影响。方法：对一名正常受试者进行了两种保湿方法(局部涂抹白色软石蜡(凡士林)和水浸泡)，对手指垫皮肤层的水分和脱水进行了详细的体内高分辨率MRI研究。这项脱水研究是在长时间皮肤保湿后立即进行的。研究了7名健康志愿者在使用凡士林0小时和7小时皮肤水化的个体间变异性(团体研究)。通过观察前臂和指垫皮肤的水化情况，研究了同一受试者皮肤水化的位置依赖性。系统稳定性和测量重复性通过详细的体模研究得到验证。结果：在体素尺寸为50×150×1000微米的情况下，获得了正常皮肤和水合皮肤的活体图像。水合和脱水作为接触保湿剂(即水和凡士林)的函数对图像信号强度、观察到的T1以及特定组织中自由水和结合水的相互作用的影响被识别出来，并与现有的生理知识相关联。水化引起的角质层肿胀表现为体内组织水化的模型。结论：脱水研究结果表明，先前皮肤水化引起的变化对所有皮肤层都是可逆的。对于这两种保湿方法(即凡士林和沐浴皮肤)，保湿和脱水对皮肤的影响是相似的。指垫和手臂皮肤的MRI参数的变化趋势相似。该小组研究显示，角质层和表皮水化的受试者间变异性较低。
Background/aims: Many claims are made as to the efficacy of topical preparations in moisturising the skin, yet most of these claims cannot be substantiated by scientific study for the skin layers beneath the stratum corneum, and yield no information on the remainder of the epidermis and dermis. This argues for an in vivo quantitative method for measuring the effect of water loading extended to various layers of the skin.Methods: Detailed high-resolution in vivo MRI studies of hydration and dehydration of finger pad skin layers were conducted on one normal subject using two moisturisation methods (topical white soft paraffin (Vaseline) and water immersion). The dehydration study was carried out immediately following removal from prolonged skin moisturisation. Inter-individual variability for skin hydration (group study) was studied in seven healthy volunteers at 0 and 7 h hydration with Vaseline. Location dependence in skin hydration was investigated on the same subject by looking into the hydration of forearm and finger pad skin. System stability and measurement reproducibility was verified through a detailed phantom study.Results: Images of normal and hydrated human skin were obtained in vivo at voxel dimensions of 50 mumx150 mumx1000 mum. The effect of hydration and dehydration as a function of exposure to moisturiser (i.e. water and Vaseline) on the image signal intensity, observed T1, and interaction of free and bound water in specific tissues were identified and correlated with existing physiological knowledge. Swelling of stratum corneum due to hydration was expressed as an in vivo model of tissue hydration.Conclusion: Results of the dehydration study showed that the changes due to the previous hydration of the skin are reversible for all skin layers. For both moisturisation methods (i.e. Vaseline and skin bathing), the effects of hydration and dehydration on the skin were similar. The trends of the MRI parameters for finger pad and arm skin were similar. The group study showed low inter-subject variability of hydration on stratum corneum and epidermis.