Cellular interactions in the generation of cytolytic T lymphocyte responses: role of Ia-positive splenic adherent cells in presentation in H-2 antigen.
Cellular interactions in the generation of cytolytic T lymphocyte responses: role of Ia-positive splenic adherent cells in presentation in H-2 antigen.
复制标题
产生溶细胞性 T 淋巴细胞反应中的细胞相互作用：Ia 阳性脾贴壁细胞在 H-2 抗原呈递中的作用。
DOI：
10.1073/pnas.77.10.6091
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发表时间：
1980
影响因子：
11.1
通讯作者：
Burakoff,SJ
中科院分区：
文献类型：
--
作者：
Weinberger,O;Herrmann,SH;Mescher,MF;Benacerraf,B;Burakoff,SJ
脾贴壁细胞是产生溶细胞性T淋巴细胞对完整刺激细胞的初级和（在限制抗原剂量下）次级同种异体应答所必需的。对纯化的同种异体质膜或脂质体中纯化的H-2Kk抗原的次级应答也依赖于脾粘附细胞。这些反应的产生需要Ia阳性的存在。脾粘附细胞群中的Thy 1，2阴性抗辐射细胞，粘附于玻璃、塑料和尼龙毛上。用与Ia+脾粘附细胞结合的纯化H-2Kk同种异体抗原刺激溶细胞性T淋巴细胞前体比用直接加入培养物的抗原刺激有效10 - 20倍。此外，观察到当向培养物中加入对响应细胞的Ia具有特异性的抗Iad抗血清时，溶细胞性T淋巴细胞对脂质体的响应显著降低。这些结果表明，纯化的蛋白质的主要组织相容性复合体，抗原呈递Ia+脾粘附细胞在产生溶细胞性T淋巴细胞反应中发挥作用。
Splenic adherent cells are required for generation of both primary and, at limiting antigen dose, secondary allogeneic responses of cytolytic T lymphocytes to intact stimulator cells. Secondary responses to purified allogeneic plasma membranes or purified H-2Kk antigens in liposomes are also dependent upon splenic adherent cells. Generation of these responses requires the presence of an Ia-positive. Thy 1,2-negative, radiation-resistant cell in the splenic adherent cell population that is adherent to glass, plastic, and nylon wool. Stimulation of cytolytic T lymphocyte precursors with purified H-2Kk alloantigen bound to Ia+ splenic adherent cells is 10-20 times more efficient than stimulation with antigen added directly to culture. Furthermore, a marked decrease in the response of cytolytic T lymphocytes to liposomes was observed when antiserum against Iad specific for the Ia of the responder cells was added to culture. These results demonstrate that, for purified proteins of the major histocompatibility complex, antigen presentation by Ia+ splenic adherent cells plays a role in the generation of a cytolytic T lymphocyte response.