Derivation of Rabbit Embryonic Stem Cells from Vitrified-Thawed Embryos.
Derivation of Rabbit Embryonic Stem Cells from Vitrified-Thawed Embryos.
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从玻璃化解冻胚胎中衍生兔胚胎干细胞。
DOI：
10.1089/cell.2015.0044
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发表时间：
2015-12
期刊：
影响因子：
--
通讯作者：
Xu Jie
中科院分区：
文献类型：
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作者：
Du Fuliang;Chen Chien-Hong;Li Yi;Hu Yeshu;An Li-You;Yang Lan;Zhang Jifeng;Chen Y Eugene;Xu Jie
兔子是再生医学的一种有用的动物模型。我们以前开发了多能兔胚胎干细胞（rbESC）线使用新鲜胚胎。我们还成功地用玻璃化法冷冻保存了兔胚胎。在目前的工作中，我们结合这两种技术来获得rbESCs使用玻璃化解冻（V/T）胚胎。我们证明，V/T囊胚（BL）可以用来获得多能rbESCs的效率与使用新鲜的BL。这些胚胎干细胞与来自新鲜胚胎的胚胎干细胞无法区分。我们通过BL注射实验检测了来自V/T胚胎的rbESCs的发育能力，并制备了嵌合试剂盒。我们的工作将冷冻保存添加到兔干细胞研究和应用的工具箱中，并将大大扩展临床相关动物模型中再生医学的可用研究材料。
The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the present work, we combined these two technologies to derive rbESCs using vitrified-thawed (V/T) embryos. We demonstrate that V/T blastocysts (BLs) can be used to derive pluripotent rbESCs with efficiencies comparable to those using fresh BLs. These ESCs are undistinguishable from the ones derived from fresh embryos. We tested the developmental capacity of rbESCs derived from V/T embryos by BL injection experiments and produced chimeric kits. Our work adds cryopreservation to the toolbox of rabbit stem cell research and applications and will greatly expand the available research materials for regenerative medicine in a clinically relevant animal model.