Templation-induced re-equilibration in polymer-scaffolded dynamic combinatorial libraries leads to enhancements in binding affinities
Templation-induced re-equilibration in polymer-scaffolded dynamic combinatorial libraries leads to enhancements in binding affinities
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DOI：
10.1039/c3sc51413g
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发表时间：
2013-07
期刊：
影响因子：
8.4
通讯作者：
Clare S. Mahon;D. Fulton
中科院分区：
文献类型：
--
作者：
Clare S. Mahon;D. Fulton
将大分子模板添加到通过将酰肼可逆缀合到双官能化聚合物支架上构建的聚合物支架动态组合文库（PS-DCL）中已显示产生对模板具有增强的结合亲和力的聚合物群体，如通过基于荧光的方法所确定的。利用文库成员和模板之间的静电相互作用的模板化过程已被证明是可逆的，其中PS-DCL在去除模板后返回其初始组成。固体支持的模板已被用于方便地分离库的最佳结合级分。
Addition of macromolecular templates to Polymer-Scaffolded Dynamic Combinatorial Libraries (PS-DCLs) constructed by the reversible conjugation of acylhydrazides onto aldehyde-functionalised polymer scaffolds has been shown to generate a population of polymers with enhanced binding affinities towards templates, as determined by a fluorescence-based method. The templating process, which harnesses electrostatic interactions between library members and templates, has been shown to be reversible, with PS-DCLs returning to their initial composition upon removal of the template. Solid-supported templates have been employed for the convenient isolation of the best-binding fraction of the library.