RodZ (YfgA) is required for proper assembly of the MreB actin cytoskeleton and cell shape in E-coli
RodZ (YfgA) is required for proper assembly of the MreB actin cytoskeleton and cell shape in E-coli
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DOI：
10.1038/emboj.2008.264
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发表时间：
2009-02-04
期刊：
影响因子：
11.4
通讯作者：
de Boer, Piet A. J.
中科院分区：
文献类型：
--
作者：
Bendezu, Felipe O.;Hale, Cynthia A.;de Boer, Piet A. J.
在大多数非球形生物体中，细菌MreB肌动蛋白细胞骨架是维持细胞形状所必需的。在杆状细胞如大肠杆菌中，它通常沿着长轴在细胞质膜下以螺旋状构型组装。这种结构是如何被控制的，以及它如何帮助决定细胞的形状还不清楚。在一个新的细胞形状突变体的遗传筛选中，我们确定了RodZ（YfgA）作为细胞骨架的重要跨膜成分。RodZ的缺失导致MreB错误组装成非螺旋结构，以及随之而来的细胞形状的丧失。RodZ的近膜结构域对维持杆形状至关重要，而膜两侧的其他结构域具有关键但部分冗余的功能。虽然这些结构域之一类似于DNA结合基序，我们的证据表明，它是主要负责协会的RodZ与细胞骨架。
The bacterial MreB actin cytoskeleton is required for cell shape maintenance in most non-spherical organisms. In rod-shaped cells such as Escherichia coli, it typically assembles along the long axis in a spiral-like configuration just underneath the cytoplasmic membrane. How this configuration is controlled and how it helps dictate cell shape is unclear. In a new genetic screen for cell shape mutants, we identified RodZ (YfgA) as an important transmembrane component of the cytoskeleton. Loss of RodZ leads to misassembly of MreB into non-spiral structures, and a consequent loss of cell shape. A juxta-membrane domain of RodZ is essential to maintain rod shape, whereas other domains on either side of the membrane have critical, but partially redundant, functions. Though one of these domains resembles a DNA-binding motif, our evidence indicates that it is primarily responsible for association of RodZ with the cytoskeleton.