Mesenchymal Stem Cell-Derived Microvesicles Support Ex Vivo Expansion of Cord Blood-Derived CD34(+) Cells.
Mesenchymal Stem Cell-Derived Microvesicles Support Ex Vivo Expansion of Cord Blood-Derived CD34(+) Cells.
复制标题
间充质干细胞衍生的微泡支持脐带血衍生的 CD34( ) 细胞的离体扩增
DOI：
10.1155/2016/6493241
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发表时间：
2016
影响因子：
4.3
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Xie H;Sun L;Zhang L;Liu T;Chen L;Zhao A;Lei Q;Gao F;Zou P;Li Q;Guo AY;Chen Z;Wang H
已知间充质干细胞（MSC）在骨髓造血微环境中支持造血干细胞和祖细胞（HSPC）的特征性质。MSC在共培养系统中用作饲养层，用于脐带血（CB）的离体扩增以增加CB中相对低数量的HSPC。越来越多的研究表明，MSC衍生的微泡（MSC-MV）在其亲本细胞的生物学功能中发挥重要作用。我们推测MSC-MV可能重现了其亲本细胞的造血支持作用。在目前的研究中，我们发现MSC-MV含有参与造血调控的microRNA。我们还证明了MSC-MVs可以促进CB来源的单个核细胞和CD 34+细胞的扩增，并在体外产生更多的原始祖细胞。此外，当MSC-MVs加入CB-MSC共培养体系中时，它们可以提高MSC的造血支持作用。这些发现突出了MSC-MVs在CB体外扩增中的作用，这可能为CB移植提供一种有前途的治疗方法。
Mesenchymal stem cells (MSCs) are known to support the characteristic properties of hematopoietic stem and progenitor cells (HSPCs) in the bone marrow hematopoietic microenvironment. MSCs are used in coculture systems as a feeder layer for the ex vivo expansion of umbilical cord blood (CB) to increase the relatively low number of HSPCs in CB. Findings increasingly suggest that MSC-derived microvesicles (MSC-MVs) play an important role in the biological functions of their parent cells. We speculate that MSC-MVs may recapitulate the hematopoiesis-supporting effects of their parent cells. In the current study, we found MSC-MVs containing microRNAs that are involved in the regulation of hematopoiesis. We also demonstrated that MSC-MVs could improve the expansion of CB-derived mononuclear cells and CD34+ cells and generate a greater number of primitive progenitor cells in vitro. Additionally, when MSC-MVs were added to the CB-MSC coculture system, they could improve the hematopoiesis-supporting effects of MSCs. These findings highlight the role of MSC-MVs in the ex vivo expansion of CB, which may offer a promising therapeutic approach in CB transplantation.