Ion concentration changes in renal cells during regulatory volume decrease.
Ion concentration changes in renal cells during regulatory volume decrease.
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调节容量减少期间肾细胞中离子浓度的变化。
DOI：
10.1152/ajprenal.1993.265.1.f77
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Rick,R
中科院分区：
文献类型：
--
作者：
Rick,R
采用电子探针微量分析来跟踪调节体积减少期间离子浓度的变化。测量是在分离的小管束上进行的，这些小管束是从新西兰白兔肾脏的髓射线中解剖出来的。通过在 190 mosM 培养基中孵育细胞束，诱导细胞肿胀和随后的调节体积减小；对照束在290 mosM 培养基中孵育。在这两种条件下，可检测离子约占细胞内渗透压的 80%。所有细胞在细胞体积调节过程中都会损失大量的 Na、K 和 Cl。虽然在近端直小管中，超过一半的离子丢失是Na和Cl，但在皮质集合管的主细胞和嵌入细胞以及粗升肢中，Na和Cl的丢失仅起到次要作用。 Na 和 K 的流出量大大超过 Cl 的流出量，表明损失了额外的不可检测的阴离子（碳酸氢根）。对细胞核、细胞质和几个细胞器的单独测量揭示了亚细胞离子分布的一些不均匀性。
Electron-probe microanalysis was employed to follow ion concentration changes during regulatory volume decrease. The measurements were performed on isolated tubule bundles, which were dissected from medullary rays of New Zealand White rabbit kidneys. Cell swelling and subsequent regulatory volume decrease were induced by incubating the bundles in 190 mosM medium; control bundles were incubated in 290 mosM medium. Under both conditions, the detectable ions accounted for approximately 80% of the intracellular osmolarity. All cells lost significant amounts of Na, K, and Cl during cell volume regulation. While in the proximal straight tubule more than one-half of the ions lost were Na and Cl, in principal and intercalated cells of the cortical collecting duct and in the thick ascending limb the losses of Na and Cl played only a minor role. The efflux of Na and K greatly exceeded the Cl efflux, suggesting the loss of an additional undetectable anion (bicarbonate). Separate measurements in the nucleus, cytoplasm, and several cellular organelles revealed some inhomogeneity of the subcellular ion distribution.