The synthesis of complex-type oligosaccharides. I. Structure of the lipid-linked oligosaccharide precursor of the complex-type oligosaccharides of the vesicular stomatitis virus G protein.
The synthesis of complex-type oligosaccharides. I. Structure of the lipid-linked oligosaccharide precursor of the complex-type oligosaccharides of the vesicular stomatitis virus G protein.
复制标题
复合型低聚糖的合成。
DOI：
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发表时间：
1978
影响因子：
4.8
通讯作者：
S. Kornfeld
中科院分区：
文献类型：
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作者：
E. Li;I. Tabas;S. Kornfeld
囊泡气孔炎病毒G蛋白的复合型寡糖单位的合成是通过将高分子量寡糖从脂质载体转移到新生多肽的。转移后，通过除去构成复合型寡糖外部分支的糖和甘露糖残基以及糖糖残基的去除来“处理”寡糖。已经阐明了脂质连接前体的寡糖部分的结构，以进一步定义加工所涉及的步骤。由于获得足够量的标准结构研究材料是不可行的，因此大多数结构研究都是对放射性标记材料进行的，放射性均匀地掺入葡萄糖，甘露糖和N-乙酰葡萄糖中。基于β-N-乙酰葡萄糖氨基酶CII消化，α-甘露糖苷酶消化，乙酸解析，史密斯周期降解，甲基化分析和周期氧化，我们提出了以下结构，以实现以下结构，以实现以下结构的脂质含量的含量烯丙基二氯的寡糖酸性含氧糖化的结构。
The synthesis of the complex-type oligosaccharide unit of the vesicular stomatitis virus G protein is initiated by the en bloc transfer of a high molecular weight oligosaccharide from a lipid carrier to the nascent polypeptide. Following transfer the oligosaccharide is "processed" by removal of glucose and mannose residues and the sugars that constitute the outer branches of the complex-type oligosaccharide are added. The structure of the oligosaccharide moiety of the lipid-linked precursor has been elucidated in order to further define the steps involved in processing. Since it was not feasible to obtain adequate amounts of material for standard structural studies, most of the structural studies were performed on radiolabeled material, with radioactivity incorporated differentially into glucose, mannose, and N-acetylglucosamine. Based on endo-beta-N-acetylglucosaminidase CII digestion, alpha-mannosidase digestion, acetolysis, Smith periodate degradation, methylation analysis, and periodate oxidation, we propose the following structure for the oligosaccharide moiety of the lipid-linked oligosaccharide.