Oocyte atresia in WT (HdrR) and P53 (-/-) medaka ( Oryzias latipes ) exposed to UVA
Oocyte atresia in WT (HdrR) and P53 (-/-) medaka ( Oryzias latipes ) exposed to UVA
复制标题
暴露于 UVA 的 WT (HdrR) 和 P53 (-/-) 青鳉 ( Oryzias latipes ) 卵母细胞闭锁
DOI：
10.1016/j.jphotobiol.2018.04.016
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Mitani Hiroshi
中科院分区：
文献类型：
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作者：
Sayed A.H.;Ismail Rania F.;Mitani Hiroshi
环境紫外线（UVA）对水环境的负面影响最近得到了强调; UVA可以通过刺激对浮游生物的压力而产生有害影响。UVA对雌鱼卵母细胞特性的影响知之甚少。本研究探讨了生态学相关水平的模拟UVA辐射对青鳉（Oryzias latipes）两个主要品系WT（HdrR）和P53（-/-）成熟雌鱼卵巢的影响。鱼被分配到控制和三个UVA暴露组（15分钟，30分钟，和60分钟/天）为三天，样品选择是24小时和14天后曝光。组织学变化和卵母细胞闭锁的百分比进行了分析，在UVA暴露的鱼相比，控制。改变包括滤泡细胞肥大伴厚度增加、卵绒毛膜破裂（辐射状）、皮质肺泡损伤以及细胞核和细胞质变形。闭锁的百分比显着增加与较高的UVA暴露剂量和时间的野生型和p53缺陷的鱼。野生型的卵母细胞闭锁率显著高于p53突变体。这些结果表明，UVA暴露引起组织学改变，在p53和WT青鳉卵母细胞导致卵泡闭锁，这降低了女性生殖能力和幼虫生产。UVA卵母细胞的反应表现出p53依赖和独立的组织学改变，然而，p53突变体是不太敏感的UVA比野生型青鳉鱼。
The negative effects of ambient ultraviolet (UVA) on the water environment have been recently highlighted; UVA can create deleterious effects by stimulating stress on pelagic organisms. Little is known about UVA effects on oocyte characteristics of female fish. In the present study we explored the effects of exposure to ecologically relevant levels of simulated UVA radiation on ovaries of two major strains WT (HdrR) and P53 (-/-) of medaka (Oryzias latipes) mature female. Fish were assigned to control and three UVA-exposed groups as (15 min, 30 min, and 60 min/day) for three days and sample selection was 24 h and 14 days after exposure. Histological alterations and oocyte atresia percentage were analyzed in the UVA-exposed fish compared to control. Alteration comprised hyperthrophied follicular cells with increased thickness, breakdown of egg chorion (zona radiata), damage of cortical alveoli, and distorted nucleus and cytoplasm. The atresia percentages significantly increased with higher UVA exposure dose and time for both the wild type and the p53 deficient fish. The wild type displayed significantly higher oocyte atresia percentage than the p53 mutant. These results suggested that UVA exposure provoked histological alterations in both p53 and WT medaka oocytes leading to follicular atresia, which reduce female reproductive ability and larval production. UVA oocyte response showed p53 dependent and independent histological alteration, however, the p53 mutant was less sensitive to UVA than the wild type in medaka fish.