Preparation of injectable hydrophilic dextran/AgNPs nanocomposite product: White light active biomolecules as an antitumor agent.
Preparation of injectable hydrophilic dextran/AgNPs nanocomposite product: White light active biomolecules as an antitumor agent.
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DOI：
10.1016/j.ijbiomac.2023.125215
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发表时间：
2023-06
影响因子：
8.2
通讯作者：
U. Bunyatova;M. Hammouda;Jennifer Zhang
中科院分区：
文献类型：
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作者：
U. Bunyatova;M. Hammouda;Jennifer Zhang
包括黑色素瘤在内的各种癌症的发病率在全球范围内继续上升。虽然近年来治疗选择有所扩大，但这些治疗的好处是许多患者的疗程较短。因此，人们非常希望有新的治疗方案。在这里，我们提出了一种将葡聚糖/反应性共聚物/AgNPs纳米复合材料与无害的可见光方法相结合的方法，以获得具有强大抗肿瘤活性的血浆替代碳水化合物纳米产品(D@AgNP)。光驱动的多糖基纳米复合材料为超小(8-12 nm)AgNPs的封端以及随后特定的自组装成球形云纳米结构提供了必要条件。得到的生物相容性D@AgNP在室温下6个月内稳定，在406 nm处有吸收峰。新配方的纳米产品对A375显示出有效的抗癌活性，24小时的IC50为0.0035 mg/mL；24小时和48小时的时间点分别为0.001 mg/mL和0.0005 mg/mL的细胞完全死亡。扫描电子显微镜观察表明，D@AgNP改变了细胞结构的形状，破坏了细胞膜。透射电子显微镜观察表明，D@AgNP主要定位于内小体、溶酶体和线粒体等囊泡中。预计引入的新方法将成为改进基于生物相容的亲水性碳水化合物抗癌药物生成的基石。
Incidence of various cancers including melanoma continues to rise worldwide. While treatment options have expanded in the recent years, the benefit of these treatments suffer from short period of duration for many patients. Hence, new treatment options are highly desired. Here, we propose a method combining a Dextran/reactive-copolymer/AgNPs nanocomposite and a harmless visible light approach to obtain a plasma substitute carbohydrate-based nanoproduct (D@AgNP) that shows strong antitumor activity. Light-driven polysaccharide-based nanocomposite provided essential conditions for extra small (8-12nm) AgNPs capping with subsequent specific self-assembly into spherical-like cloud nanostructures. Obtained biocompatible D@AgNP are stable over six months at room temperature and demonstrated absorbance peak at 406 nm. New formulated nanoproduct revealed efficient anticancer properties against A375 with IC50 0.0035 mg/mL following 24-h incubation; complete cell death is achieved at 0.001 mg/mL and 0.0005 mg/mL by 24- and 48-h time points, respectively. SEM examination shows that D@AgNP altered the shape of the cell structure and damaged the cell membrane. TEM finding shows that D@AgNP are mostly localized at vesicles such as the endosomes, lysosomes and mitochondria. It is anticipated that the introduced new method serves as the cornerstone for improving the generation of biocompatible hydrophilic carbohydrate-based anticancer drugs.