Interleukin-1 beta (IL-1 beta) and tumour necrosis factor (TNF) inhibit long-term potentiation in the rat dentate gyrus in vitro
Interleukin-1 beta (IL-1 beta) and tumour necrosis factor (TNF) inhibit long-term potentiation in the rat dentate gyrus in vitro
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DOI：
10.1016/0304-3940(95)12252-4
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发表时间：
1996-01-12
影响因子：
2.5
通讯作者：
OConnor, JJ
中科院分区：
文献类型：
--
作者：
Cunningham, AJ;Murray, CA;OConnor, JJ
研究了细胞因子白介素 1 β (IL-1 β) 及其受体拮抗剂 IL-1ra 对大鼠海马齿状回的长期增强作用。在齿状回分子区域的细胞外记录场兴奋性突触后电位，以响应内侧穿通路径的刺激。低频突触传递不受 IL-1 β (1 ng/ml) 的影响，但用 IL-1 β 预处理完全阻断长时程增强的诱导。联合应用IL-1β和IL-1ra (100ng/ml)减弱了IL-1β的抑制作用。与这些发现同时，我们证明 IL-1 beta 也抑制 Ca-45 流入切片。 IL-1β对诱导的抑制作用通过肿瘤坏死因子(TNF；4.5ng/ml)和脂多糖(LPS；10μg/ml)来模拟。这些结果表明海马细胞因子的调节作用，并表明 IL-1β 对长时程增强的抑制作用可能与其对钙通道活性的抑制作用有关。
The effects of the cytokine, interleukin-1 beta (IL-1 beta), and its receptor antagonist IL-1ra, were studied on long-term potentiation in the dentate gyrus of rat hippocampal slices. Field excitatory postsynaptic potentials were recorded extracellularly in the molecular region of the dentate gyrus in response to stimulation of the medial perforant path. Low frequency synaptic transmission was unaffected by IL-1 beta (1 ng/ml), but pre-treatment with IL-1 beta completely blocked induction of long-term potentiation. Co-application of IL-1 beta and IL-1ra (100 ng/ml) attenuated the inhibitory effect of IL-1 beta. In parallel with these findings, we demonstrate that IL-1 beta also inhibited Ca-45 influx into the slices. The inhibitory effect of IL-1 beta on induction was mimicked by tumour necrosis factor (TNF; 4.5 ng/ml) and lipopolysaccharide (LPS; 10 mu g/ml). These results indicate a modulatory role for cytokines in hippocampus and suggest that the inhibitory effect of IL-1 beta on long-term potentiation may relate to its inhibitory effect on calcium channel activity.