Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle
Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle
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DOI：
10.1152/ajpheart.00201.2002
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发表时间：
2002-09-01
影响因子：
4.8
通讯作者：
Miller, VM
中科院分区：
文献类型：
--
作者：
Bracamonte, MP;Rud, KS;Miller, VM
实验旨在确定卵巢切除术如何调节血小板中的促有丝分裂因子以及这些因子如何影响冠状动脉平滑肌的增殖。通过 ELISA 对来自性腺完整和卵巢切除的成年雌性猪的血小板裂解物和贫血小板血浆中的血小板源性生长因子（PDGF（AB）和 PDGF（BB））、转化生长因子（TGF-β（1）和 TGF-β（2））和血管内皮生长因子（VEGF（165））进行定量。测定两组猪培养的冠状动脉平滑肌细胞（SMC）对自体或异体血小板裂解物的反应的增殖情况。随着卵巢切除术，PDGF(BB) 的血小板浓度增加，但 PDGF(AB)、TGF-β(1) 和 TGF-β(2) 浓度不增加。两组血小板中均未检测到 VEGF165。暴露于自体或异源血小板裂解物时，来自卵巢切除雌性的 SMC 的增殖明显大于来自完整雌性的 SMC 的增殖。这些结果表明卵巢切除术增加了血小板中 PDGF(BB) 的浓度。血小板中较高水平的 PDGF(BB) 与其他血小板衍生产品协同作用，可能有助于增强增殖性动脉对卵巢切除术后损伤的反应。
Experiments were designed to determine how ovariectomy modulates mitogenic factors in platelets and how these factors affect proliferation of coronary arterial smooth muscle. Platelet-derived growth factors (PDGF(AB) and PDGF(BB)), transforming growth factors (TGF-beta(1) and TGF-beta(2)), and vascular endothelial growth factor (VEGF(165)) were quantified in platelet lysates and platelet-poor plasma from adult gonadally intact and ovariectomized female pigs by ELISA. Proliferation of cultured coronary arterial smooth muscle cells (SMCs) from both groups of pigs was determined in response to autologous or heterologous platelet lysates. Platelet concentrations of PDGF(BB), but not PDGF(AB), TGF-beta(1), and TGF-beta(2), increased with ovariectomy. VEGF165 was not detected in platelets from either group. Proliferation of SMCs from ovariectomized females was significantly greater on exposure to autologous or heterologous platelet lysates than proliferation of SMCs from intact females. These results indicate that ovariectomy increases concentrations of PDGF(BB) in platelets. Higher levels of PDGF(BB) in platelets in synergy with other platelet-derived products could contribute to increased proliferative arterial response to injury after ovariectomy.