Corticotropin releasing factor (CRF) modulates fear-induced alterations in sleep in mice.
Corticotropin releasing factor (CRF) modulates fear-induced alterations in sleep in mice.
复制标题
促肾上腺皮质激素释放因子 (CRF) 调节小鼠恐惧引起的睡眠变化。
DOI：
10.1016/j.brainres.2009.04.017
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发表时间：
2009
期刊：
影响因子：
2.9
通讯作者：
Sanford,LarryD
中科院分区：
文献类型：
--
作者：
Yang,Linghui;Tang,Xiangdong;Wellman,LaurieL;Liu,Xianling;Sanford,LarryD
背景恐惧显著减少小鼠和大鼠在暴露后睡眠期间的快速眼动睡眠（REM）。促肾上腺皮质激素释放因子（CRF）在中枢神经系统应激反应中起重要作用。我们研究了CRF和非特异性CRF拮抗剂astressin（AST）对BALB/c小鼠情境恐惧后睡眠的影响。雄性小鼠被植入通过遥测记录睡眠的发射器和瞄准侧脑室的引导套管。在ICV微量注射生理盐水（SAL）后，然后暴露于新的室中，获得溶剂和处理对照的记录。之后，使小鼠经受电击训练（20次试验，0.5mA，0.5s持续时间）2个阶段。训练后，在暴露于单独的休克环境之前，单独的小鼠组接受SAL（0.2 μl，n=9）、CRF（0.4 μg，n=8）或AST（1.0 μg，n=8）的ICV显微注射。然后记录20小时的睡眠（8小时光照和12小时黑暗期）。与处理对照组相比，背景恐惧显著降低了接受SAL的小鼠和接受CRF的小鼠在8小时光照期间的REM，但在接受AST的小鼠中没有。接受CRF的小鼠表现出减少REM在12小时黑暗期后的背景恐惧，而小鼠接受SAL或AST没有。CRF还降低了EEG中非REM（NREM）δ（慢波）振幅。只有在背景恐惧之前接受SAL的小鼠表现出NREM和总睡眠的显著减少。这些发现表明，中央CRF系统在调节由背景恐惧引起的睡眠改变中的作用。
Contextual fear significantly reduces rapid eye movement sleep (REM) during post-exposure sleep in mice and rats. Corticotropin releasing factor (CRF) plays a major role in CNS responses to stressors. We examined the influence of CRF and astressin (AST), a non-specific CRF antagonist, on sleep after contextual fear in BALB/c mice. Male mice were implanted with transmitters for recording sleep via telemetry and with a guide cannula aimed into the lateral ventricle. Recordings for vehicle and handling control were obtained after ICV microinjection of saline (SAL) followed by exposure to a novel chamber. Afterwards, the mice were subjected to shock training (20 trials, 0.5 mA, 0.5 s duration) for 2 sessions. After training, separate groups of mice received ICV microinjections of SAL (0.2 μl, n=9), CRF (0.4 μg, n=8), or AST (1.0 μg, n=8) prior to exposure to the shock context alone. Sleep was then recorded for 20 h (8-hour light and 12-hour dark period). Compared to handling control, contextual fear significantly decreased REM during the 8-h light period in mice receiving SAL and in mice receiving CRF, but not in the mice receiving AST. Mice receiving CRF exhibited reductions in REM during the 12-h dark period after contextual fear, whereas mice receiving SAL or AST did not. CRF also reduced non-REM (NREM) delta (slow wave) amplitude in the EEG. Only mice receiving SAL prior to contextual fear exhibited significant reductions in NREM and total sleep. These findings demonstrate a role for the central CRF system in regulating alterations in sleep induced by contextual fear.