Proteomic analysis of early melanosomes: Identification of novel melanosomal proteins
Proteomic analysis of early melanosomes: Identification of novel melanosomal proteins
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DOI：
10.1021/pr025562r
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发表时间：
2003-01-01
影响因子：
4.4
通讯作者：
Appella, E
中科院分区：
文献类型：
--
作者：
Basrur, V;Yang, F;Appella, E
黑色素是一种非均质生物聚合物，仅由称为黑素细胞的特定细胞产生，黑素细胞合成色素并将其存款在称为黑素体的专门的膜结合细胞器中。虽然黑素体被怀疑与溶酶体和血小板密切相关，但黑素体蛋白的总数仍然未知。到目前为止，已经确定了六个黑素体特异性蛋白质，挑战是表征黑素体的完整蛋白质组，以进一步了解其生物发生的机制。在这份报告中，我们使用质谱和亚细胞分离，以确定早期黑素体的蛋白质成分。使用这种方法，我们已经确定了所有6个已知的黑素体特异性蛋白质，56个蛋白质与其他细胞器共享，并确认存在6个新的黑素体蛋白质，使用蛋白质印迹和免疫组织化学。
Melanin is a heterogeneous biopolymer produced only by specific cells termed melanocytes, which synthesize and deposit the pigment in specialized membrane-bound organelles known as melanosomes. Although melanosomes have been suspected of being closely related to lysosomes and platelets, the total number of melanosomal proteins is still unknown. Thus far, six melanosome-specific proteins have been identified, and the challenge is to characterize the complete proteome of the melanosome to further understand its mechanism of biogenesis. In this report, we used mass spectrometry and subcellular fractionation to identify protein components of early melanosomes. Using this approach, we have identified all 6 of the known melanosome-specific proteins, 56 proteins that are shared with other organelles, and confirmed the presence of 6 novel melanosomal proteins using western blotting and by immunohistochemistry.