Elevated expression of IL-17RB and ST2 on myeloid dendritic cells is associated with a Th2-skewed eosinophilic inflammation in nasal polyps.
Elevated expression of IL-17RB and ST2 on myeloid dendritic cells is associated with a Th2-skewed eosinophilic inflammation in nasal polyps.
复制标题
骨髓树突状细胞上 IL-17RB 和 ST2 表达升高与鼻息肉中 Th2 偏向的嗜酸性粒细胞炎症相关。
DOI：
10.1186/s13601-018-0237-4
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Sun Yueqi
中科院分区：
文献类型：
--
作者：
Zheng Rui;Wang Dan;Wang Kai;Gao Wen-Xiang;Yang Qin-Tai;Jiang Li-Jie;Zhou Min;Cao Yu-Jie;Shi Jianbo;Sun Yueqi
背景白细胞介素（IL）-25、IL-33和胸腺基质淋巴细胞生成素（TSLP）参与了Th2应答过程中上皮细胞和树突状细胞（DC）之间的相互作用。本研究旨在检测鼻息肉（NP）中髓样DC上IL-17 RB、IL-25、IL-33和TSLP受体ST2和TSLPR的表达，并评估其与NP患者局部Th2炎症和疾病严重程度的相关性。采用定量聚合酶链反应（qPCR）检测NP和对照组织中细胞因子TSLP、IL-25和IL-33以及干扰素（IFN）-γ、IL-4、IL-5、IL-13和IL-17 A的mRNA表达。IL-17 RB，ST2和TSLPR的表达以及其他表面标志物对髓样DCs（mDCs）进行了检查，通过流式细胞术。mDCs表达IL-17RB、ST2和TSLPR均高于对照组。活化的mDCs在NP中表达上调OX40 L和ICOSL，下调PDL 1。此外，在NP中，mDC上的IL-17RB、ST2和TSLPR水平分别与IL-25、IL-33和TSLP mRNA水平呈正相关。此外，IL-17 RB和ST 2在mDC上的表达与NP中IL-5 mRNA水平以及嗜酸性粒细胞数目相关。重要的是，IL-17 RB的表达和OX40 L的表达活化的mDCs在NP与CT评分和总鼻症状scores.Conclusionsincreased表达IL-17 RB和ST2的mDCs与增强局部Th2炎症NP，提示mDCs可能发挥作用，在IL-25和IL-33诱导的2型反应和嗜酸性粒细胞炎症NP。
BackgroundInterleukin(IL)-25, IL-33, and thymic stromal lymphopoietin (TSLP) underlie the crosstalk between epithelial cells and dendritic cells (DCs) during the development of Th2 responses. This study aimed to measure the expressions of IL-17RB, ST2 and TSLPR, receptor of IL-25, IL-33, and TSLP respectively, on myeloid DCs in nasal polyps (NP) and evaluate their association with local Th2 inflammation and disease severity in patients with NP.MethodsSamples were collected from 30 NP patients and 16 control subjects recruited prospectively. The mRNA expression of cytokines, including TSLP, IL-25 and IL-33, as well as interferon (IFN)-γ, IL-4, IL-5, IL-13 and IL-17A in NP and control tissues was examined by qualitative polymerase chain reaction (qPCR). The expression of IL-17RB, ST2 and TSLPR as well as other surface markers on myeloid DCs (mDCs) was examined by flow cytometry.ResultsIncreased numbers of total and activated mDCs were found in NP patients. mDCs demonstrated significantly higher expression of IL-17RB, ST2 and TSLPR than those in control tissues. The activated mDCs exhibited up-regulations of OX40L and ICOSL, but down-regulation of PDL1 in NP. Moreover, the IL-17RB, ST2 and TSLPR levels on mDCs were positively correlated with IL-25, IL-33 and TSLP mRNA levels, respectively, in NP. Furthermore, IL-17RB and ST2 expressions on mDCs were correlated with the IL-5 mRNA level as well as eosinophil number in NP. Importantly, the IL-17RB expression on mDCs and the OX40L expression on activated mDCs in NP were positively correlated with CT score and total nasal symptom score.ConclusionsIncreased expressions of IL-17RB and ST2 on mDCs are associated with enhanced local Th2 inflammation in NP, suggesting that mDCs might play a role in IL-25- and IL-33-induced type 2 responses and eosinophilic inflammation in NP.