The Arabidopsis UDP-glycosyltransferase75B1, conjugates abscisic acid and affects plant response to abiotic stresses
The Arabidopsis UDP-glycosyltransferase75B1, conjugates abscisic acid and affects plant response to abiotic stresses
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拟南芥 UDP-糖基转移酶75B1，结合脱落酸并影响植物对非生物胁迫的反应
DOI：
10.1007/s11103-019-00953-4
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发表时间：
2020-03-01
影响因子：
5.1
通讯作者：
Li, Yan-jie
中科院分区：
文献类型：
--
作者：
Chen, Ting-Ting;Liu, Fang-Fei;Li, Yan-jie
关键信息本研究揭示了拟南芥UGT 75 B1在逆境胁迫下通过糖基化调节阿坝活性的重要作用。先前的分析已经显示了UGT 75 B1在体外对阿坝的活性。然而，UGT 75 B1的生物学作用仍有待阐明。在这里，我们通过阿坝糖基化来表征UGT 75 B1在非生物胁迫响应中的功能。GUS检测和qRT-PCR结果表明，UGT 75 B1在渗透胁迫、盐胁迫和阿坝胁迫下表达量显著增加。UGT 75 B1在拟南芥中的过表达导致更高的种子发芽率和幼苗绿化率暴露于盐和渗透胁迫。相比之下，bigUGT 75 B1过表达植株在盐和渗透胁迫下更敏感。此外，UGT 75 B1过表达植株在干旱条件下表现出更大的气孔开度和更多的水分损失，这可以通过在UGT 75 B1 OE植株中检测到的响应于水分亏缺条件的较低的阿坝水平来解释。UGT 75 B1的异位表达导致许多ABA响应基因在逆境条件下表达下调，包括新萌发幼苗中的ABI 3、ABI 5和大株中的RD 29 A、KIN 1、AIL 1。总之，我们的研究结果表明，拟南芥UGT 75 B1通过阿坝的糖基化在应对非生物胁迫中起着重要作用。
Key messageThis study revealed that the Arabidopsis UGT75B1 plays an important role in modulating ABA activity by glycosylation when confronting stress environments.AbstractThe cellular ABA content and activity can be tightly controlled in several ways, one of which is glycosylation by family 1 UDP-glycosyltransferases (UGTs). Previous analysis has shown UGT75B1 activity towards ABA in vitro. However, the biological role of UGT75B1 remains to be elucidated. Here, we characterized the function of UGT75B1 in abiotic stress responses via ABA glycosylation. GUS assay and qRT-PCR indicated thatUGT75B1is significantly upregulated by adverse conditions, such as osmotic stress, salinity and ABA. Overexpression ofUGT75B1in Arabidopsis leads to higher seed germination rates and seedling greening rates upon exposure to salt and osmotic stresses. In contrast, the bigUGT75B1overexpression plants are more sensitive under salt and osmotic stresses. Additionally, theUGT75B1overexpression plants showed larger stomatal aperture and more water loss under drought condition, which can be explained by lower ABA levels examined inUGT75B1OE plants in response to water deficit conditions. Consistently,UGT75B1ectopic expression leads to downregulation of many ABA-responsive genes under stress conditions, includingABI3, ABI5newly germinated seedlings andRD29A,KIN1,AIL1in big plants. In summary, our results revealed that the Arabidopsis UGT75B1 plays an important role in coping with abiotic stresses via glycosylation of ABA.