Concentration of osteoprotegerin (OPG) in peritoneal fluid is increased in women with endometriosis
Concentration of osteoprotegerin (OPG) in peritoneal fluid is increased in women with endometriosis
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DOI：
10.1093/humrep/deh412
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发表时间：
2004-10-01
影响因子：
6.1
通讯作者：
Taketani, Y
中科院分区：
文献类型：
--
作者：
Harada, M;Osuga, Y;Taketani, Y
背景技术背景：腹腔内回流的子宫内膜细胞凋亡失败可能是子宫内膜异位症发生的一个病因。骨保护素（OPG）是一种生存因子，通过与肿瘤坏死因子（TNF）相关的凋亡诱导配体（TRAIL）结合，从而阻止TRAIL与凋亡受体DR 4和DR 5结合来发挥其作用。在本研究中，我们讨论了TRAIL/OPG系统参与子宫内膜异位症发病机制的可能性。方法：采用特异性酶联免疫吸附法检测子宫内膜异位症患者和非子宫内膜异位症患者腹腔液（PF）中OPG和TRAIL的浓度。采用逆转录-聚合酶链反应（RT-PCR）检测DR 4和DR 5在乳腺癌组织中的表达。结果：子宫内膜异位症患者PF中OPG浓度显著高于非子宫内膜异位症患者（P=0.006）。就疾病的分期而言，III/IV期子宫内膜异位症患者的OPG浓度显著高于无子宫内膜异位症患者和I/II期子宫内膜异位症患者。另一方面，III/IV期子宫内膜异位症的TRAIL/OPG浓度比值显著低于非子宫内膜异位症和I/II期子宫内膜异位症。DR 5 mRNA在所有研究的组织中均有表达。结论：这些结果表明，TRAIL/OPG系统参与了子宫内膜异位症的病理生理过程，可能影响了子宫内膜异位症细胞的凋亡。
BACKGROUND: Failure of apoptosis of refluxed endometrial cells within the peritoneal cavity is a possible etiologic factor for development of endometriosis. Osteoprotegerin (OPG) is a survival factor that exerts its effect by binding to tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), thus preventing TRAIL from binding to the apoptosis receptors DR4 and DR5. In the present study, we addressed the possibility that the TRAIL/OPG system is involved in the pathogenesis of endometriosis. METHODS: Concentrations of OPG and TRAIL in the peritoneal fluid (PF) of women with or without endometriosis were measured using specific enzyme-linked immunoabsorbent assay. The expression of DR4 and DR5 in the endometriotic tissue was examined by reverse transcription-polymerase chain reaction. RESULTS: OPG concentrations in PF of women with endometriosis were significantly higher than those of women without endometriosis (P=0.006). With respect to the stages of the disease, the concentrations of OPG in women with stage III/IV endometriosis were significantly higher than in those without endometriosis and those with stage I/II endometriosis. On the other hand, the ratios of TRAIL/OPG concentrations were significantly lower in stage III/IV endometriosis compared to those in non-endometriosis and stage I/II endometriosis. DR5 mRNA expression was clearly detected in all the endometriotic tissues studied. CONCLUSIONS: These findings suggest that the TRAIL/OPG system is involved in the pathophysiology of endometriosis, possibly affecting the apoptosis of endometriotic cells.