Single nucleotide polymorphism typing on DNA array with hydrophobic surface fabricated by plasma-polymerization technique
Single nucleotide polymorphism typing on DNA array with hydrophobic surface fabricated by plasma-polymerization technique
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DOI：
10.1016/j.bios.2004.02.022
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发表时间：
2004-09-15
影响因子：
12.6
通讯作者：
Karube, I
中科院分区：
文献类型：
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作者：
Miyachi, H;Ikebukuro, K;Karube, I
一个DNA阵列已被制作在玻璃基板上，这使得高通量分析的单碱基错配。本工作采用微加工相容的等离子体聚合法固定探针DNA，通过改变探针DNA的表面性质来研究探针DNA的杂交行为。在基底上构建由35埃PP层组成的固定化基质，其额外施加在链霉亲和素吸收的六甲基二硅氧烷（HMDS）-PP层上，以将生物素化的DNA探针锚在表面上。与其亲水性乙腈-PP层相比，疏水性固定基质被认为可以提高杂交的准确性和效率。在HMDS-PP表面制备的寡核苷酸芯片可有效检测ApoE基因的单核苷酸多态性（single nucleotide polymorphism，SNPs）。(C)2004 Elsevier B. V.保留所有权利。
A DNA array has been fabricated on glass substrates, which enables high-throughput analysis of single-base mismatches. In this work, microfabrication-compatible plasma-polymerization (PP) method was used for immobilizing probe DNAs to study the hybridization behavior by changing surface properties. The immobilization matrix consisting of 35 Angstrom of PP layer, applied additionally on the streptavidin absorbed hexamethyldisiloxane (HMDS)-PP layer, was constructed on the substrates to anchor biotinylated DNA probes onto the surface. The hydrophobic immobilization matrix was considered to enhance hybridization accuracy and efficiency, compared with its hydrophilic acetonitrile-PP layers. The oligonucleotide arrays fabricated on HMDS-PP surface were shown to be effective in detection of single nucleotide polymorphisms (SNPs) of ApoE gene. (C) 2004 Elsevier B.V. All rights reserved.