Comparison of the performance of the rapid antigen detection actim Influenza A&B test and RT-PCR in different respiratory specimens
Comparison of the performance of the rapid antigen detection actim Influenza A&B test and RT-PCR in different respiratory specimens
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DOI：
10.1099/jmm.0.004358-0
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发表时间：
2009-03-01
影响因子：
3
通讯作者：
Koenig, B.
中科院分区：
文献类型：
--
作者：
Ghebremedhin, B.;Engelmann, I.;Koenig, B.
如今，流感抗原检测试剂盒最常用于检测甲型或B型流感病毒，以快速建立流感诊断并开始适当的治疗。进行本研究以评价actim Influenza A&B test（Meidium Biochemica）的性能。总体而言，在actin，甲型和B流感检测中分析了473份呼吸道标本，并将结果与RT-PCR检测结果进行了比较;其中461份样本来自7周至6.5岁的有流感相关症状或重症监护室的儿科患者，12份样本来自有潜在肺部或血液病的成人。使用actim甲型和B型流感病毒检测可确定甲型或B型流感病毒的诊断（9/473份样本为甲型流感病毒，6/473份样本为B型流感病毒）。RT-PCR结果显示23例患者携带流感病毒（13/473为甲型流感病毒，10/473为B型流感病毒）。与RT-PCR法相比，ACTIM甲型和B型流感病毒检测的敏感性和特异性分别为65%和100%。然而，32个室间质评样品含有7种不同的甲型流感亚型H1N1和H3 N2和禽流感H5 N1的正确检出的actim甲型和B型流感测试。未观察到与一系列细菌、真菌和其他病毒病原体的交叉反应性。总之，ACTIM甲型和B型流感病毒检测试剂盒由于其高度特异性，对于阳性结果是可靠的。然而，由于诊断样本的灵敏度较低，因此该检测的阴性结果需要通过更灵敏的检测方法进行确认。
Nowadays, influenza antigen detection test kits are used most frequently to detect influenza A or B virus to establish the diagnosis of influenza rapidly and initiate appropriate therapy. This study was conducted to evaluate the performance of the actim Influenza A&B test (Medix Biochemica). Overall, 473 respiratory specimens were analysed in the actim, Influenza A&B test and the results were compared with those from an RT-PCR assay; 461 of these samples originated from paediatric patients aged 7 weeks to 6.5 years either with influenza-related symptoms or from the intensive care unit, and 12 samples originated from adults with underlying lung or haematological diseases. Diagnosis of influenza A or B virus could be established using the actim Influenza A&B test (9/473 samples for influenza A virus and 6/473 for influenza B virus). RT-PCR revealed 23 patients with influenza virus (13/473 for influenza A virus and 10/473 for influenza B virus). The sensitivity and specificity of the actim Influenza A&B test were 65 and 100% compared with the RT-PCR assay. However, 32 external quality assessment samples containing seven different strains of influenza A subtypes H1N1 and H3N2 and the avian H5N1 were detected correctly by the actim Influenza A&B test. No cross-reactivity to a range of bacterial, fungal and other viral pathogens was observed. In conclusion, the actim Influenza A&B test is reliable for positive results due to its high specificity. Nevertheless, negative results from this test need to be confirmed by a more sensitive assay because of the low sensitivity observed with diagnostic samples.