Transcriptome analysis of secondary-wall-enriched seed coat tissues of canola (Brassica napus L.)
Transcriptome analysis of secondary-wall-enriched seed coat tissues of canola (Brassica napus L.)
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DOI：
10.1007/s00299-010-0824-x
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发表时间：
2010-04-01
影响因子：
6.2
通讯作者：
Deyholos, Michael K.
中科院分区：
文献类型：
--
作者：
Jiang, Yuanqing;Deyholos, Michael K.
甘蓝型油菜（Brassica napus）的种皮来源于胚珠珠被，并且含有一层栅栏细胞，其具有厚的次生壁。由于种皮的纤维素壁和其他不可消化的成分对油料种子的价值有负面影响，因此正在努力改变种子发育。为了促进这些努力，并更好地了解种皮的生物学，我们使用了90，000个元素的微阵列来鉴定其转录本在发育中的B种皮中表达的基因。油菜。将种皮分成三层，并将中间部分（包含富含栅栏的组织和大量内珠被）的转录表达与发育中的下胚轴的转录表达进行比较后，我们鉴定了674个基因，其转录本在中间部分中更丰富。种皮。其中包括种皮身份的特征标记和许多与细胞壁多糖，类黄酮和各种细胞壁蛋白质和转录因子代谢相关的基因。相反，我们发现了1，203个基因，与种皮相比，这些基因的转录物在下胚轴组织中更丰富，包括木质部特异性标记，例如XCP 1和XCP 2。我们使用定量RT-PCR验证了21个差异表达的转录本。结果定义了一组转录本，其在B的发育种皮中高度富集。油菜。
The seed coat of Brassica napus (canola, oilseed rape) is derived from ovule integuments and contains a layer of palisade cells, which have thick secondary walls. Because cellulosic walls and other indigestible components of the seed coat contribute negatively to the value of oilseeds, efforts are underway to alter seed development. To facilitate these efforts, and to better understand the biology of seed coats, we used a 90,000 element microarray to identify genes whose transcripts were expressed in developing seed coats of B. napus. After dissecting seed coats into three layers, and comparing transcript expression in the middle fraction (which contained the palisade-enriched tissue and bulk of inner integument) to transcript expression in developing hypocotyls, we identified 674 genes whose transcripts were more abundant in the middle fraction of the seed coat. Among these were well-characterized markers of seed coat identity and many genes associated with metabolism of cell wall polysaccharides, flavonoids and various cell wall proteins and transcription factors. Conversely, we identified 1,203 genes whose transcripts were more abundant in the hypocotyl tissue as compared to seed coat, including xylem-specific markers, such as XCP1 and XCP2. We validated 21 of the differentially expressed transcripts using quantitative RT-PCR. The results define a set of transcripts that are highly enriched in the developing seed coat of B. napus.