Genotyping of Porphyromonas gingivalis in Relationship to Virulence
Genotyping of Porphyromonas gingivalis in Relationship to Virulence
复制标题
牙龈卟啉单胞菌的基因分型与毒力的关系
DOI：
10.1007/978-1-0716-0939-2_6
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发表时间：
2020
影响因子：
--
通讯作者：
Takeuchi Hiroki
中科院分区：
文献类型：
--
作者：
Amano Atsuo;Choi Youn-Hee;Takeuchi Hiroki
牙龈卟啉单胞菌是一种重要的牙周病原体，已知其具有与其毒力相关的遗传变异。此外，由thefimA基因编码的菌毛参与细菌对宿主细胞的粘附和侵袭，并且是细菌的已知毒力因子。 ThefimA 基因分为六种变体（I-V 型和 Ib 型），并已被证明与微生物毒力相关。聚合酶链式反应 (PCR) 检测结果有助于区分基因型，一些研究人员已开发出用于区分基因型的特定引物组。尽管仍有改进的空间，但基因分型有望成为牙周检查和诊断的有用技术。在本章中，目前可用的PCR方法对P的基因型变异进行分类。描述了牙龈。
Porphyromonas gingivalis, a significant periodontal pathogen, is known to possess genetic variations in relation to its virulence. Furthermore, fimbriae encoded by thefimAgene are involved in bacterial adherence to and invasion of host cells, and a known virulence factor of the bacterium. ThefimAgene is classified into six variants (types I–V and Ib) and has been shown to be related to microbial virulence. Polymerase chain reaction (PCR) assay results are helpful to differentiate the genotypes, withfimAtype-specific primer sets used for that have been developed by several researchers. Although room for improvement remains,fimAgenotyping is expected to become a useful technique for periodontal examinations and diagnosis. In this chapter, currently available PCR methods to classifyfimAgenotypic variations ofP. gingivalisare described.