CELL-SURFACE PROTEIN ANTIGEN FROM WOLINELLA-RECTA ATCC-33238T
CELL-SURFACE PROTEIN ANTIGEN FROM WOLINELLA-RECTA ATCC-33238T
复制标题
DOI：
10.1128/jcm.27.6.1210-1217.1989
复制
发表时间：
1989-06-01
影响因子：
9.4
通讯作者：
MURAYAMA, Y
中科院分区：
文献类型：
--
作者：
KOKEGUCHI, S;KATO, K;MURAYAMA, Y
通过酸提取法从直窝螺细胞表面选择性地分离出一种高分子量（约150，000）的蛋白质，并通过在DEAE-纤维素上的负吸附和凝胶过滤进行纯化。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明，该蛋白存在于W。而其它Wolinella种如W. curva和W.产琥珀酸菌牙周炎患者的血清与这种蛋白抗原反应强烈，而健康供体的血清显示很少或没有反应，如免疫印迹分析所确定的。在血清中，免疫球蛋白G抗体的蛋白抗原的滴度显着高于牙周炎患者比牙周健康的捐助者，通过酶联免疫吸附试验检测。
A high-molecular-weight (approximately 150,000) protein was selectively isolated by acid extraction from the cell surface of Wolinella recta and purified by negative adsorption on DEAE-cellulose and gel filtration. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that this protein was found in W. recta but not in other Wolinella species, such as W. curva and W. succinogenes. Sera from patients with periodontitis reacted strongly with this protein antigen, whereas sera from healthy donors showed little or no reactivity, as determined by immunoblotting analysis. In serum, titers of immunoglobulin G antibodies to the protein antigen were significantly higher in patients with periodontitis than in periodontally healthy donors, as detected by an enzyme-linked immunosorbent assay.