Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla locus on barley chromosome 1H
Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla locus on barley chromosome 1H
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DOI：
10.1007/bf02172376
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发表时间：
1996-06-24
期刊：
影响因子：
--
通讯作者：
Wise, RP
中科院分区：
文献类型：
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作者：
DeScenzo, RA;Wise, RP
脉冲场凝胶电泳技术的变体与二维DNA凝胶电泳（2-DDGE）一起使用，以确定物理遗传距离的比例在两个遗传定义的间隔大麦染色体1H。2-DDCE分析表明，两个位点，定义了0.3 cM的间隔，通过与BCD 249杂交确定，驻留在一个单一的450 kb的MluI片段。这一结果表明，与大麦基因组整体的3.7-4.2 Mb/cM相比，在1500 kb/cM的区间内物理距离与遗传距离的最大比率。用NotI限制并依次用MWG 068和BCD 249探测的高分子量（HMW）DNA在C. I中产生约2.5 Mb和3.0 Mb的弥散条带。16151和C.I. 16155个亲本系。这些结果表明，在由这些探针限定的区间内，物理距离与遗传距离的最大比率为7.5Mb/cM。独特的HMW DNA限制性片段长度多态性（RFLP）归因于重组断点的存在。来自重组断点分析的数据用于估计Xbcd249.2-Xmwg 068区间中的2.5Mb/cM的物理与遗传距离的比率和Xbcd-349.1-Xbcd249.2区间中的0.465Mb/cM的物理与遗传距离的比率。物理连锁和重组断裂点分析表明，Xbcd249.1-Xbcd249.2区间在物理上比Xbcd249.2-Xmwg 068区间小约5倍。
Variants of the pulsed-field gel electrophoresis technique were used in conjunction with two-dimensional DNA gel electrophoresis (2-DDGE) to determine the ratio of physical to genetic distance in two genetically defined intervals on barley chromosome 1H. 2-DDCE analysis demonstrated that two loci that define a 0.3 cM interval, as determined by hybridization with BCD249, reside on a single 450-kb MluI fragment. This result indicates a maximum ratio of physical to genetic distance in this interval of 1500 kb/cM as compared to 3.7-4.2 Mb/cM for the barley genome as a whole. High molecular weight (HMW) DNA restricted with NotI and probed sequentially with MWG068 and BCD249 yield diffuse bands at approximately 2.5 Mb and 3.0 Mb in the C.I. 16151 and C.I. 16155 parental lines, respectively. These results suggest the maximum ratio of physical to genetic distance in the interval defined by these probes is 7.5 Mb/cM. Unique HMW DNA restriction fragment length polymorphisms (RFLP) were attributed to the presence of recombination breakpoints. Data from the recombination breakpoint analysis were used to estimate a ratio of physical to genetic distance of 2.5 Mb/cM in the Xbcd249.2-Xmwg068 interval and 0.465 Mb/cM in the Xbcd-349.1-Xbcd249.2 interval. Both physical linkage and recombination breakpoint analysis indicate the Xbcd249.1-Xbcd249.2 interval is approximately five-fold smaller, physically, than the Xbcd249.2-Xmwg068 interval.