The activation of antiviral RNA interference not only exists in neural progenitor cells but also in somatic cells in mammals.
The activation of antiviral RNA interference not only exists in neural progenitor cells but also in somatic cells in mammals.
复制标题
抗病毒RNA干扰的激活不仅存在于神经祖细胞中，也存在于哺乳动物的体细胞中。
DOI：
10.1080/22221751.2020.1787798
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Li Yang
中科院分区：
文献类型：
--
作者：
Zhang Yuqiang;Li Zhe;Ye Zhi;Xu Yan;Wang Binbin;Wang Congcong;Dai Yunpeng;Lu Jinfeng;Lu Boxun;Zhang Wanju;Li Yang
RNA干扰（RNAi）途径是植物和无脊椎动物抗病毒免疫的重要机制。最近，我们和其他人已经证明，抗病毒RNAi反应在哺乳动物中也是保守的，至少对五种不同的RNA病毒，包括寨卡病毒（ZIKV）。ZIKV可以优先感染发育中的胎儿脑中的神经元祖细胞（NPC）JIKV离体感染在人NPC中诱导RNAi介导的抗病毒应答，但在更分化的NPC或体细胞中不诱导RNAi介导的抗病毒应答。然而，关于靶向ZIKV的病毒衍生的小干扰RNA（vsiRNA）的体内特性或功能知之甚少。在这里，我们报告了一个令人惊讶的观察结果：与体内观察不同，靶向ZIKV的病毒小RNA（vsRNA）在中枢神经系统（CNS）和肌肉组织中的体内感染中产生。此外，我们的研究结果表明，在抗病毒RNAi激活辛德毕斯病毒（SINV）后，在小鼠CNS中产生典型的vsiRNA，这表明哺乳动物在CNS中应用抗病毒免疫策略的可能性。
The RNA interference (RNAi) pathway directs an important antiviral immunity mechanism in plants and invertebrates. Recently, we and others have demonstrated that the antiviral RNAi response is also conserved in mammals, at least to five distinct RNA viruses, including Zika virus (ZIKV). ZIKV may preferentially infect neuronal progenitor cells (NPCs) in the developing foetal brain.Ex vivoZIKV infection induces RNAi-mediated antiviral response in human NPCs, but not in the more differentiated NPCs or somatic cells. However, litter is known about thein vivoproperty or function of the virus-derived small-interfering RNAs (vsiRNAs) targeting ZIKV. Here we report a surprising observation: different fromex vivoobservations, viral small RNAs (vsRNAs) targeting ZIKV were producedin vivoupon infection in both central neuron system (CNS) and muscle tissues. In addition, our findings demonstrate the production of canonical vsiRNAs in murine CNS upon antiviral RNAi activation by Sindbis virus (SINV), suggesting the possibility of antiviral immune strategy applied by mammals in the CNS.