Comparison of MAP and tyrosine kinase signaling in heterophils from commercial and wild-type turkeys
Comparison of MAP and tyrosine kinase signaling in heterophils from commercial and wild-type turkeys
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DOI：
10.1016/j.dci.2006.12.002
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发表时间：
2007-01-01
影响因子：
2.9
通讯作者：
Kogut, Michael H.
中科院分区：
文献类型：
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作者：
Genovesea, Kenneth J.;He, Haiqi;Kogut, Michael H.
蛋白酪氨酸和促分裂原活化激酶是宿主先天免疫应答的关键介质，将信号从宿主细胞上的表面受体传递到细胞核，在细胞核中发生基因表达。在孵化后第4天和第7天，从野生型格兰德河火鸡和商业火鸡品系（品系A）中分离嗜异性粒细胞。用沙门氏菌（SE）或调理素化SE（OPSE）刺激嗜异性粒细胞1小时。刺激后，裂解细胞并测试裂解物的促分裂原活化蛋白激酶（MAPK）活性。具体地，使用市售的ELISA试剂盒测定细胞外信号调节蛋白激酶（ERK 1/2）的磷酸化和p38 MAPK磷酸化。总蛋白酪氨酸激酶（PTK）活性也进行了测定。在孵化后第4天和第7天，来自格兰德河火鸡的异嗜性细胞在用SE或OPSE刺激后具有显著更高水平的ERK 1/2和p38 MAPK激酶活性（p < 0.001）。同样，格兰德河火鸡嗜异性细胞在第4天和第7天的PTK值在SE刺激后显著高于OPSE刺激，并且显著高于OPSE刺激后的PTK值（p
Protein tyrosine and mitogen-activated kinases are crucial mediators of the host innate immune response, conferring signals from surface receptors on the host cell to the nucleus of the cell, where gene expression occurs. Heterophils were isolated from wild-type Rio Grande turkeys and a commercial line of turkeys (Line A) on days 4 and 7 post-hatch. Heterophils were stimulated for 1 h with Salmonella enteritidis (SE) or opsonized SE (OPSE). After stimulation, cells were lysed and lysates were tested for mitogen-activated protein kinase (MAPK) activity. Specifically, phosphorylation of extracellular signal-regulated protein kinase (ERK1/2) and p38 MAPK phosphorylation were assayed using commercially available ELISA kits. Total protein tyrosine kinase (PTK) activity was also assayed. On both days 4 and 7 post-hatch, heterophils from Rio Grande turkeys had significantly higher levels of ERK1/2 and p38 MAPK kinase activity upon stimulation with either SE or OPSE (p < 0.001). Likewise, PTK values on days 4 and 7 in Rio Grande turkey heterophils were significantly higher upon stimulation with SE than with OPSE and were significantly (p