Gene Expression Profiles Induced by High-dose Ionizing Radiation in MDA-MB-231 Triple-negative Breast Cancer Cell Line
Gene Expression Profiles Induced by High-dose Ionizing Radiation in MDA-MB-231 Triple-negative Breast Cancer Cell Line
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DOI：
10.21873/cgp.20130
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发表时间：
2019-07-01
影响因子：
2.5
通讯作者：
Forte, Giusi Irma
中科院分区：
文献类型：
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作者：
Bravata, Valentina;Cammarata, Francesco Paolo;Forte, Giusi Irma
背景/目的：放射治疗（RT）是乳腺癌（BC）的一种治疗选择。即使大量的BC患者接受RT，也不是所有人都报告受益，因为辐射抗性通过几个因素被激活，例如激素受体状态。在此，我们分析了三阴性BC（TNBC）MDA-MB-231中RT诱导的基因表达谱（GEP），以研究参与辐射抗性的信号传导网络。材料与方法：采用基因芯片技术，对高剂量辐射处理的MDA-MB-231 BC细胞的GEP进行分析。此外，为了检查RT诱导的细胞效应，还进行了形态学分析和克隆形成评价。结果：从我们的芯片数据中报告了IR诱导的GEP和通路的描述性报告。此外，选择的MDA-MB-231抗辐射细胞组分（RCF）包括能够驱动辐射抗性的特定分子。结论：总之，我们的数据突出了TNBC MDA-MB-231细胞系在转录水平上的RT响应，就激活这些细胞中的辐射抗性而言，作为晚期BC的模型。
Background/Aim: Radiation therapy (RT) represents a therapeutic option in breast cancer (BC). Even if a great number of BC patients receive RT, not all of them report benefits, due to radioresistance that gets activated through several factors, such as the hormone receptor status. Herein, we analyzed the gene expression profiles (GEP) induced by RT in triple-negative BC (TNBC) MDA-MB-231, to study signalling networks involved in radioresistance. Materials and Methods: GEP of MDA-MB-231 BC cells treated with a high dose of radiation, went through cDNA microarray analysis. In addition, to examine the cellular effects induced by RT, analyses of morphology and clonogenic evaluation were also conducted. Results: A descriptive report of GEP and pathways induced by IR is reported from our microarray data. Moreover, the MDA-MB-231 Radioresistent Cell Fraction (RCF) selected, included specific molecules able to drive radioresistance. Conclusion: In summary, our data highlight, the RT response of TNBC MDA-MB-231 cell line at a transcriptional level, in terms of activating radioresistance in these cells, as a model of late-stage BC.