Probiotic lactobacilli mediated changes in global epigenetic signatures of human intestinal epithelial cells during Escherichia coli challenge
Probiotic lactobacilli mediated changes in global epigenetic signatures of human intestinal epithelial cells during Escherichia coli challenge
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DOI：
10.1007/s13213-019-01451-0
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发表时间：
2019-06-01
影响因子：
3
通讯作者：
Kapila, Rajeev
中科院分区：
文献类型：
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作者：
Bhat, Mohd Iqbal;Kumari, Ankita;Kapila, Rajeev
宿主基因组环境关联对于个体的正常发育和功能发挥至关重要。细胞附近的微生物群和益生菌可以提供这样的关键刺激，其可以引起不同的表观遗传介导的宿主反应。本研究的目的是探索Caco-2细胞的表观遗传特征的变化由益生菌菌株（鼠李糖乳杆菌MTCC 5897：LR，发酵乳杆菌MTCC 5898：LF和它们的混合物：RF），分别，或在挑战与大肠杆菌（ATCC 14948）使用排除，竞争和置换试验。用LR、LF、RF和E处理腺癌肠上皮Caco-2细胞。coli中培养6 h。Caco-2细胞用E.大肠杆菌和益生菌乳酸杆菌在排斥，竞争和置换试验。最后，通过H4和H3组蛋白的乙酰化和DNA甲基化模式确定全局表观遗传修饰。益生菌处理的Caco-2细胞表现出显著的（p
Host genome environment association is critical for proper development and functioning of an individual. Microbiota and probiotics within the cellular vicinity may serve such critical stimuli that can bring out different epigenetic mediated host responses. The aim of present study was to explore the changes in epigenetic signatures of Caco-2 cells by probiotic strains (Lactobacillus rhamnosus MTCC 5897: LR, Lactobacillus fermentum MTCC 5898: LF and their mixture: RF), respectively, or during challenge with Escherichia coli (ATCC 14948) using exclusion, competition and displacement assays. Adenocarcinoma intestinal epithelial Caco-2 cells were treated with LR, LF, RF and E. coli for 6h, respectively. Caco-2 cells were also challenged with E. coli and probiotic lactobacilli during exclusion, competition and displacement assays. Finally, global epigenetic modifications by acetylation of H4 and H3 histone proteins and DNA methylation patterns were determined. Probiotic-treated Caco-2 cells displayed significant (p