Mutually exclusive sense-antisense transcription at FLC facilitates environmentally induced gene repression.
Mutually exclusive sense-antisense transcription at FLC facilitates environmentally induced gene repression.
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DOI：
10.1038/ncomms13031
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发表时间：
2016-10-07
影响因子：
16.6
通讯作者：
Dean, Caroline
中科院分区：
文献类型：
--
作者：
Rosa, Stefanie;Duncan, Susan;Dean, Caroline
通过基因区进行反义转录在大多数基因组中普遍存在；然而，其功能意义尚不清楚。我们正在研究反义转录本（COOLAIR）在拟南芥开花位点C （FLC）冷诱导的表观遗传沉默中的作用，FLC是向生殖过渡的调节因子。在这里，我们使用单分子RNA FISH在细胞水平上解决FLC和COOLAIR转录的机制关系。我们证明，虽然义转录本和反义转录本可以在同一细胞中共存，但它们在单个位点上是互斥的。在越来越多的细胞中，寒冷强烈上调COOLAIR转录，并通过互斥关系促进顺式关闭sense FLC转录。COOLAIR转录本在每个位点形成密集云，通过改变H3K36me3动力学影响FLC转录。这些结果可能对其他显示正义和反义转录的基因座具有普遍意义。基因区反义转录是一种普遍现象。在这里，Rosa等人使用单分子FISH表明，在拟南芥春化过程中，冷诱导的COOLAIR反义转录与来自同一位点的开花调节因子FLC的义链转录是互斥的。
Antisense transcription through genic regions is pervasive in most genomes; however, its functional significance is still unclear. We are studying the role of antisense transcripts (COOLAIR) in the cold-induced, epigenetic silencing of Arabidopsis FLOWERING LOCUS C (FLC), a regulator of the transition to reproduction. Here we use single-molecule RNA FISH to address the mechanistic relationship of FLC and COOLAIR transcription at the cellular level. We demonstrate that while sense and antisense transcripts can co-occur in the same cell they are mutually exclusive at individual loci. Cold strongly upregulates COOLAIR transcription in an increased number of cells and through the mutually exclusive relationship facilitates shutdown of sense FLC transcription in cis. COOLAIR transcripts form dense clouds at each locus, acting to influence FLC transcription through changed H3K36me3 dynamics. These results may have general implications for other loci showing both sense and antisense transcription. Antisense transcription from genic regions is a common phenomenon. Here Rosa et al. use single molecule FISH to show that during vernalization in Arabidopsis, cold-induced antisense transcription of COOLAIR is mutually exclusive to sense-strand transcription of the flowering regulator FLC from the same loci.
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影响因子：
48
作者：
Raj, Arjun;van den Bogaard, Patrick;Rifkin, Scott A.;van Oudenaarden, Alexander;Tyagi, Sanjay
通讯作者：
Tyagi, Sanjay
影响因子：
5.1
作者：
Duncan S;Olsson TSG;Hartley M;Dean C;Rosa S
通讯作者：
Rosa S
DOI：
10.1073/pnas.1419030111
发表时间：
2014-11-11
影响因子：
11.1
作者：
Csorba, Tibor;Questa, Julia I.;Dean, Caroline
通讯作者：
Dean, Caroline
影响因子：
11.6
作者：
Michaels, SD;Amasino, RM
通讯作者：
Amasino, RM
DOI：
10.1073/pnas.1406130112
发表时间：
2015-04-07
影响因子：
11.1
作者：
Li, Na;Joska, Tammy M.;Belden, William J.
通讯作者：
Belden, William J.