Radiosensitivity of testicular cells in the fetal mouse.
Radiosensitivity of testicular cells in the fetal mouse.
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胎鼠睾丸细胞的放射敏感性。
DOI：
10.2307/3579091
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发表时间：
1995
影响因子：
3.4
通讯作者：
Dirk G. de Rooij
中科院分区：
文献类型：
--
作者：
R. Vergouwen;R. Huiskamp;R. J. Bas;H. L. Roepers;J. Davids;Dirk G. de Rooij
本文研究了产前X射线照射对CBA/P小鼠睾丸出生后发育的影响。在交配后第14、15、18天，孕鼠接受0.25~1.5GyX射线单次照射。较高的剂量会导致胎鼠大量死亡。分别于产后3d和31d取雄性子代，用分离法测定生殖细胞数、A型精原细胞数和支持细胞数。此外，在性交后第15天照射后，还在产后3天和31天测定睾丸的间质细胞、间充质细胞、巨噬细胞、肌样细胞、淋巴管内皮细胞、内皮细胞和血管周围细胞的数量。产后第3天，照射后第14天和第15天生殖细胞数量减少。在性交后第14天测定生殖细胞放射敏感性的D0值为0.7Gy.在性交后第15天，性细胞的D0值被确定为0.8Gy值，然而，这似乎不太准确。性交后第18天的性细胞没有准确的D0值。在产后第31天，随着受照小鼠年龄的增加，生精上皮的再生量以及睾丸重量和管径受照射的影响更大。照射后第15天和第18天，显示精子细胞的管状横切面百分率随照射剂量的增加而减少，而照射后第14天，显示精子细胞的管状横切面百分率不随照射剂量的增加而减少。此外，在显示精子细胞的管状横断面上，与性交后第15天暴露于相同剂量的睾丸相比，在性交后第18天暴露于1.25和1.5Gy射线的睾丸导致每个横截面上的精子细胞数量显著减少。这表明生殖细胞对辐射的敏感性随着胎龄的增加而增加。产前照射对睾丸支持细胞数量和间质细胞类型无明显影响。结果表明，在胎鼠睾丸中，精原干细胞对X射线的敏感性高于成年睾丸，而支持细胞和间质细胞对X射线的敏感性相对较高。
The effects of prenatal X irradiation on postnatal development of the CBA/P mouse testis was studied. At days 14, 15 and 18 post coitus pregnant female mice were exposed to single doses of X rays ranging from 0.25-1.5 Gy. Higher doses resulted in extensive loss of fetal mice. In the male offspring, at days 3 and 31 post partum, the numbers of gonocytes, type A spermatogonia and Sertoli cells per testis were determined using the disector method. Furthermore, after irradiation at day 15 post coitus, the numbers of Leydig cells, mesenchymal cells, macrophages, myoid cells, lymphatic endothelial cells, endothelial cells and perivascular cells per testis were also determined at days 3 and 31 post partum. At day 3 post partum, the number of germ cells was decreased after irradiation at days 14 and 15 post coitus. A D0 value of 0.7 Gy was determined for the radiosensitivity of the gonocytes at day 14 post coitus. A D0 value of 0.8 Gy was determined for the gonocytes at day 15 post coitus which, however, seems to be less accurate. No accurate D0 value could be determined for the gonocytes at day 18 post coitus. At day 31 post partum, the repopulation of the seminiferous epithelium as well as testis weights and tubular diameters were more affected by irradiation with increasing age of the mice at the time of irradiation. The percentage of tubular cross sections showing spermatids decreased with increasing dose after irradiation at days 15 and 18 post coitus, but not after irradiation at day 14 post coitus. Furthermore, in tubular cross sections showing spermatids, exposure of testes to 1.25 and 1.5 Gy at day 18 post coitus resulted in significantly lower numbers of spermatids per cross section when compared to those testes exposed to the same doses at day 15 post coitus. This indicates that the radiosensitivity of the gonocytes increases with fetal age. Prenatal irradiation did not cause significant changes in the numbers per testis of the Sertoli cells or the interstitial cell types. The present results indicate that, in the fetal mouse testis, the spermatogonial stem cells are more sensitive to X irradiation than in the adult testis, while Sertoli cells and interstitial cells are relatively resistant.
影响因子：
4.8
作者：
HARDY, MP;ZIRKIN, BR;EWING, LL
通讯作者：
EWING, LL
垂体切除术和生精功能的改变对成年大鼠间质细胞体积、数量和增殖的影响。
影响因子：
--
作者：
Keeney,DS;Ewing,LL
通讯作者：
Ewing,LL