Activities of Aeromonas hydrophila hemolysins and their interaction with erythrocyte membranes
Activities of Aeromonas hydrophila hemolysins and their interaction with erythrocyte membranes
复制标题
嗜水气单胞菌溶血素的活性及其与红细胞膜的相互作用
作者：
S. Kozaki;K. Katō;T. Asao;Y. Kamata;G. Sakaguchi
从环境来源分离的嗜水气单胞菌 CA-11 产生的溶血素活性比从腹泻病例分离的 AH-1 菌株产生的溶血素活性对温度更敏感。 CA-11溶血素在10℃以下不能引起溶血。免疫印迹分析表明，即使没有发生溶血，两种溶血素也会在兔红细胞膜中形成寡聚体。这表明结合和随后的寡聚反应与温度无关。水杨酸钠抑制两种溶血素对兔红细胞的溶解，但选定的单糖和寡糖则不然。薄层免疫染色表明，两种溶血素均与带净负电荷的磷酸甘油酯结合，但与不带净负电荷的磷酸甘油酯结合较弱。鞘磷脂和溶血磷酸甘油酯都不与溶血素反应，而溶血素与游离脂肪酸结合。这些结果表明，溶血素与膜成分（可能是磷脂）的结合需要极性头部基团的负电荷和非极性尾部适当的疏水性。
The activity of hemolysin produced by Aeromonas hydrophila CA-11, isolated from an environmental source, was more sensitive to temperature than that of hemolysin produced by strain AH-1, isolated from a diarrheal case. CA-11 hemolysin failed to elicit hemolysis below 10 degrees C. Immunoblotting analyses showed that both hemolysins formed into oligomers in rabbit erythrocyte membrane even when no hemolysis occurred. suggesting that the binding and the subsequent oligomerization are temperature independent. Sodium salicylate inhibited lysis of rabbit erythrocytes by both hemolysins, but selected monosaccharides and oligosaccharides did not. Thin-layer immunostaining indicated that both hemolysins bound to phosphoglycerides with net negative charge but weakly to the ones with no net negative charge. Neither sphingomyelin nor lysophosphoglyceride reacted with the hemolysins, whereas the hemolysins bound to free fatty acids. These results suggest that the binding of either hemolysin to the membrane component, probably phospholipid, requires both negative charge of the polar head group and suitable hydrophobicity of the nonpolar tails.