Photoelectrochemical Imaging System for the Mapping of Cell Surface Charges
Photoelectrochemical Imaging System for the Mapping of Cell Surface Charges
复制标题
用于细胞表面电荷绘图的光电化学成像系统
DOI：
10.1021/acs.analchem.9b00304
复制
发表时间：
2019-05-07
影响因子：
7.4
通讯作者：
Krause, Steffi
中科院分区：
文献类型：
--
作者：
Wu, Fan;Zhou, Bo;Krause, Steffi
细胞表面电荷影响细胞信号、细胞代谢过程、表面黏附和细胞增殖。我们对膜电荷的作用的了解是有限的，因为我们无法在不干扰细胞或其膜的化学或物理的情况下观察到变化。在此，我们报道了一种基于无标记交流光电流测量的光电化学成像系统(PEIS)，该系统可以用于绘制生理条件下单个活细胞的基面电荷。细胞在ITO基质上培养。在外加偏置电压下，通过扫描穿过ITO镀膜玻璃基板背面的聚焦、调制的激光束来产生光电流图像。研究表明，光电流对单个活细胞基底面的负表面电荷很敏感--这是其他电化学或电生理成像技术无法触及的区域。用PEIS监测骨髓间充质干细胞的裂解情况。
The surface charge of cells affects cell signaling, cell metabolic processes, adherence to surfaces, and cell proliferation. Our understanding of the role of membrane charges is limited due to the inability to observe changes without interfering, chemically or physically, with the cell or its membrane. Here, we report that a photo-electrochemical imaging system (PEIS) based on label-free ac-photo-current measurements at indium tin oxide (ITO) coated glass substrates can be used to map the basal surface charge of single live cells under physiological conditions. Cells were cultured on the ITO substrate. Photocurrent images were generated by scanning a focused, modulated laser beam across the back of the ITO coated glass substrate under an applied bias voltage. The photocurrent was shown to be sensitive to the negative surface charge of the substrate facing, basal side of a single living cell-an area not accessible to other electrochemical or electrophysiological imaging techniques. The PEIS was used to monitor the lysis of mesenchymal stem cells.