Genetic control of compound leaf development in the mungbean (Vigna radiata L.)
Genetic control of compound leaf development in the mungbean (Vigna radiata L.)
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绿豆（Vigna radiata L.）复叶发育的遗传控制
DOI：
10.1038/s41438-018-0088-0
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发表时间：
2019-02-01
影响因子：
8.7
通讯作者：
Luo, Da
中科院分区：
文献类型：
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作者：
Jiao, Keyuan;Li, Xin;Luo, Da
许多研究表明，在豆科植物的不同分支中，复叶的发育有不同的调控过程。LEAFY（LFY）直系同源物功能的丧失导致了反向重复缺失进化枝（IRLC）和非IRLC物种中叶复杂性的不同程度降低。为了进一步了解LFY同源基因在非IRLC植物复叶发育中的作用及其分子机制，我们研究了绿豆（Vigna radiata L.）其显示复叶完全转化为单叶。我们的分析表明，UN编码绿豆LFY直系同源物（VrLFY），并在叶片发育中发挥重要作用。原位RNA杂交结果表明，STM类基因KNOXI在绿豆复叶原基中表达。此外，在绿豆中，七叶小叶1（hel1）突变体的小叶数量增加被证明依赖于VrLFY和KNOXI基因的功能。我们的研究结果表明，HEL1是一个关键因素协调不同的过程中控制绿豆及其相关的非IRLC豆类复叶发育。
Many studies suggest that there are distinct regulatory processes controlling compound leaf development in different clades of legumes. Loss of function of the LEAFY (LFY) orthologs results in a reduction of leaf complexity to different degrees in inverted repeat-lacking clade (IRLC) and non-IRLC species. To further understand the role of LFY orthologs and the molecular mechanism in compound leaf development in non-IRLC plants, we studied leaf development in unifoliate leaf (un) mutant, a classical mutant of mungbean (Vigna radiata L.), which showed a complete conversion of compound leaves into simple leaves. Our analysis revealed that UN encoded the mungbean LFY ortholog (VrLFY) and played a significant role in leaf development. In situ RNA hybridization results showed that STM-like KNOXI genes were expressed in compound leaf primordia in mungbean. Furthermore, increased leaflet number in heptafoliate leaflets1 (hel1) mutants was demonstrated to depend on the function of VrLFY and KNOXI genes in mungbean. Our results suggested that HEL1 is a key factor coordinating distinct processes in the control of compound leaf development in mungbean and its related non-IRLC legumes.