Guided neurite elongation and Schwann cell invasion into magnetically aligned collagen in simulated peripheral nerve regeneration
Guided neurite elongation and Schwann cell invasion into magnetically aligned collagen in simulated peripheral nerve regeneration
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DOI：
10.1006/exnr.1999.7095
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发表时间：
1999-08-01
影响因子：
5.3
通讯作者：
Tranquillo, RT
中科院分区：
文献类型：
--
作者：
Dubey, N;Letourneau, PC;Tranquillo, RT
在I型胶原单体自组装成原纤维的过程中，利用高强度磁场将胶原凝胶排列成直径为4毫米的棒状。我们开发了一种体外实验，研究将背根神经节（DRG）外植体放置在磁性排列的胶原凝胶棒上的神经突伸长。从鸡胚DRG神经元到这些杆状体的神经突延伸深度明显大于对照组，并且随着磁场强度的增加而增加，胶原凝胶杆状体的双折射也是如此，表明胶原纤维沿着杆状体轴线排列。此外，随着磁场强度的增加，神经突伸长的轴向偏倚变得更加明显，这可能是由于神经突尖端生长锥的接触引导响应。用双免疫标记法研究了新生大鼠DRG雪旺细胞的共侵袭。在没有血清的情况下，雪旺细胞与延长的神经突高度相关，并且经常拖尾。在血清存在的情况下，雪旺细胞的侵袭率明显较高，并形成轴向排列的弦，使人想起Bungner带。这些结果可能转化为一种改进的管状修复方法，通过充满磁性排列的胶原凝胶的管来引导和刺激轴突生长，(C) 1999学术出版社。
High-strength magnetic fields were used to align collagen gel formed into 4-mm-diameter rods during the self-assembly of type I collagen monomers into fibrils. We developed an in vitro assay to study neurite elongation into the magnetically aligned collagen gel rods from dorsal root ganglia (DRG) explants placed onto one end of the rods. The depth of neurite elongation from chick embryo DRG neurons into these rods was found to be substantially greater than that observed in controls and increased with an increase in magnetic field strength, as did the collagen gel rod birefringence, indicative of collagen fibril alignment along the rod axis. Moreover, the axial bias of neurite elongation became more pronounced with an increase in magnetic field strength, presumably due to a contact guidance response of growth cones at the neurite tips. Coinvasion of Schwann cells from neonatal rat DRG was also studied in these assays using double immunolabeling. In the absence of serum, Schwann cells were highly associated with, and often trailed, elongating neurites. In the presence of serum, Schwann cells showed significantly higher rates of invasion and formed axially aligned chords reminiscent of bands of Bungner. These results may translate into an improved method of entubulation repair of transected peripheral nerves by directing and stimulating axonal growth through a tube filled with magnetically aligned collagen gel, (C) 1999 Academic Press.