Identification of molecular determinants for the nuclear import of pseudorabies virus UL31.
Identification of molecular determinants for the nuclear import of pseudorabies virus UL31.
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DOI：
10.1016/j.abb.2015.09.024
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发表时间：
2015-12
影响因子：
3.9
通讯作者：
Meili Li;Si Jiang;C. Mo;Z. Zeng;Xiaowei Li;Chunke Chen;Yanjia Yang;Jin-lin Wang;Jinlu Huang-Jinlu-Hua
中科院分区：
文献类型：
--
作者：
Meili Li;Si Jiang;C. Mo;Z. Zeng;Xiaowei Li;Chunke Chen;Yanjia Yang;Jin-lin Wang;Jinlu Huang-Jinlu-Hua
单纯疱疹病毒1型（HSV-1）UL 31是一种多功能蛋白质，对于HSV-1感染很重要。伪狂犬病病毒（PRV）UL 31是与HSV-1 UL 31同源的晚期蛋白。先前的研究表明PRV UL 31主要定位于细胞核，然而，迄今为止，其核输入的分子决定因素尚不清楚。在此，通过利用活细胞荧光显微镜，与增强的黄色荧光蛋白融合的UL 31在活细胞中瞬时表达，并证实在不存在其他病毒蛋白的情况下仅靶向细胞核。此外，发现UL 31的核输入依赖于Ran-、输入素α1-、α3-、α5-、α7-、β1-和转运素1-介导的途径。因此，这些结果将开辟新的途径，描绘在PRV感染的UL 31的生物学功能。
Herpes simplex virus 1 (HSV-1) UL31 is a multifunctional protein and important for HSV-1 infection. Pseudorabies virus (PRV) UL31 is a late protein homologous to HSV-1 UL31. Previous studies showed that PRV UL31 is predominantly localized to nucleus, however, the molecular determinants for its nuclear import were unclear to date. Here, by utilizing live cells fluorescent microscopy, UL31 fused with enhanced yellow fluorescent protein was transiently expressed in live cells and confirmed to exclusively target to the nucleus in the absence of other viral proteins. Furthermore, the nuclear import of UL31 was found to be dependent on the Ran-, importin α1-, α3-, α5-, α7-, β1-and transportin-1-mediated pathway. Therefore, these results would open up new avenues for depicting the biological functions of UL31 during PRV infection.