TaNF-YC11, one of the light-upregulated NF-YC members in Triticum aestivum, is co-regulated with photosynthesis-related genes
TaNF-YC11, one of the light-upregulated NF-YC members in Triticum aestivum, is co-regulated with photosynthesis-related genes
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DOI：
10.1007/s10142-010-0158-3
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发表时间：
2010-01
影响因子：
2.9
通讯作者：
Troy J. Stephenson;Troy J. Stephenson;C. McIntyre;C. Collet;G. Xue
中科院分区：
文献类型：
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作者：
Troy J. Stephenson;Troy J. Stephenson;C. McIntyre;C. Collet;G. Xue
核因子Y （NF-Y）是一种异三聚体转录因子复合物。植物中每个NF-Y亚基（NF-YA、NF-YB和NF-YC）都由多个基因编码。RT-PCR分析结果显示，5个小麦NF-YC成员（TaNF-YC5、8、9、11和12）在叶片和幼苗茎部均受到光照上调。对Affymetrix小麦基因组阵列数据集的共表达分析显示，在三到四个独立的Affymetrix阵列数据集中，大量基因的转录水平与tanf - yc11和tanf - yc8基因的转录水平一致。tanf - yc11相关转录本在基因本体术语光合作用中显著富集。对tanf - yc11相关基因启动子的序列分析显示，存在假定的NF-Y复合物结合位点（CCAAT基序）。对一个潜在的TaNF-YC11靶基因亚群进行定量RT-PCR分析发现，13个基因中有10个基因在叶片和幼苗茎中都有光上调，并且与TaNF-YC11具有显著相关的表达谱。TaNF-YC11的潜在靶基因包括太阳能转化为化学能所需的所有四种类囊体膜结合复合物的亚基成员和卡尔文循环中的限速酶。这些数据表明，TaNF-YC11可能参与光合作用相关基因的调控。
Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor complex. Each of the NF-Y subunits (NF-YA, NF-YB and NF-YC) in plants is encoded by multiple genes. Quantitative RT-PCR analysis revealed that five wheat NF-YC members (TaNF-YC5,8,9,11and12) were upregulated by light in both the leaf and seedling shoot. Co-expression analysis of Affymetrix wheat genome array datasets revealed that transcript levels of a large number of genes were consistently correlated with those of theTaNF-YC11andTaNF-YC8genes in three to four separate Affymetrix array datasets.TaNF-YC11-correlated transcripts were significantly enriched with the Gene Ontology termphotosynthesis. Sequence analysis in the promoters ofTaNF-YC11-correlated genes revealed the presence of putative NF-Y complex binding sites (CCAAT motifs). Quantitative RT-PCR analysis of a subset of potential TaNF-YC11 target genes showed that ten out of the 13 genes were also light-upregulated in both the leaf and seedling shoot and had significantly correlated expression profiles withTaNF-YC11. The potential target genes for TaNF-YC11 include subunit members from all four thylakoid membrane-bound complexes required for the conversion of solar energy into chemical energy and rate-limiting enzymes in the Calvin cycle. These data indicate that TaNF-YC11 is potentially involved in regulation of photosynthesis-related genes.