DEVELOPMENT AND CHARACTERIZATION OF EST-SSR MARKERS IN THE INVASIVE WEED MIKANIA MICRANTHA (ASTERACEAE)
DEVELOPMENT AND CHARACTERIZATION OF EST-SSR MARKERS IN THE INVASIVE WEED MIKANIA MICRANTHA (ASTERACEAE)
复制标题
入侵杂草薇甘菊（菊科）EST-SSR 标记的开发和表征。
DOI：
10.3732/ajb.1000387
复制
发表时间：
2011-01-01
影响因子：
3
通讯作者：
Shi, Suhua
中科院分区：
文献类型：
--
作者：
Yan, Yubin;Huang, Yelin;Shi, Suhua
本研究的目的：利用微卫星标记研究入侵植物薇甘菊的入侵历史。转录组测序计划为新标记的开发提供了一个潜在的来源。方法和结果：从36份M.薇甘菊每个位点的等位基因数为2 ~ 4个，平均2.7个。预期杂合度和观察杂合度分别为0 ~ 0.7754和0 ~ 0.8333。结论：这些EST-SSR标记可为进一步研究黑木耳的群体遗传学奠定基础。薇甘菊作为追踪其入侵历史的额外工具。
Premise of the study : Microsatellite markers were developed for the invasive species Mikania micrantha to investigate its invasion history. Transcriptome sequencing projects offer a potential source for the development of new markers.Methods and Results : Sixteen polymorphic simple sequence repeat (SSR) markers derived from ESTs were identified and screened in 36 samples of M. micrantha. The number of alleles per locus ranged from two to four, with an average of 2.7 alleles per locus. The expected heterozygosity and observed heterozygosity ranged from 0 to 0.7754 and 0 to 0.8333, respectively.Conclusions : These EST-SSR markers may be useful for further investigation of the population genetics of M. micrantha as additional tools to trace its invasion history.