Novel Peptides from Skins of Amphibians Showed Broad-Spectrum Antimicrobial Activities
Novel Peptides from Skins of Amphibians Showed Broad-Spectrum Antimicrobial Activities
复制标题
来自两栖动物皮肤的新型肽显示出广谱抗菌活性。
DOI：
10.1111/cbdd.12672
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发表时间：
2016-03-01
影响因子：
3
通讯作者：
Zhang, Yun
中科院分区：
文献类型：
--
作者：
Wang, Ying;Zhang, Yue;Zhang, Yun
肽制剂通常被认为是开发新药的潜在生物材料，可以克服病原微生物对经典抗生素治疗日益增强的耐药性。肽制剂的一个关键来源是两栖动物的皮肤，因为它们提供了大量天然存在的抗菌肽（AMP）模板，等待进一步开发和利用。在这项研究中，使用 5 PCR 引物从 3 种蛙类青蛙（Rana limnocharis、R. exilispinosa 和 Amolops afghanus）的皮肤中鉴定出 12 种新型 AMP。共有11个AMP与目前已知的AMP家族表现出相似性，包括brevinin-1、brevinin-2、esculentin-1和nigrocin，此外，一种名为Limnochariin的AMP代表了一个新的AMP家族。所有12个AMP均含有C端环状基序，其中大多数对18种标准和临床分离的细菌菌株显示出明显的抗菌活性，其中包括4种革兰氏阳性菌、11种革兰氏阴性菌和3种真菌。这些发现提供了有用的见解，可用于抗感染肽制剂的设计。
Peptide agents are often considered as potential biomaterials for developing new drugs that can overcome the rising resistance of pathogenic micro-organisms to classic antibiotic treatments. One key source of peptide agents is amphibian skin, as they provide a great deal of naturally occurring antimicrobial peptide (AMP) templates awaiting further exploitation and utilization. In this study, 12 novel AMPs from the skins of 3 ranid frogs, Rana limnocharis, R. exilispinosa, and Amolops afghanus, were identified using a 5 PCR primer. A total of 11 AMPs exhibited similarities with currently known AMP families, including brevinin-1, brevinin-2, esculentin-1, and nigrocin, besides, one AMP, named as Limnochariin, represented a novel AMP family. All 12 AMPs contain a C-terminus cyclic motif and most of them show obvious antimicrobial activities against 18 standard and clinically isolated strains of bacteria, including 4 Gram-positive bacteria, 11 Gram-negative bacteria, and 3 fungus. These findings provide helpful insight that will be useful in the design of anti-infective peptide agents.